Planta Med 2020; 86(13/14): 983-987
DOI: 10.1055/a-1102-1168
Biological and Pharmacological Activity
Original Papers

Mogroside V-producing Endophytic Fungi Isolated from Siraitia grosvenorii

Chen Bin
1   Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China
2   College of Environment and Resource, Guangxi Normal University, Guilin, China
,
Yu Fangming
1   Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China
2   College of Environment and Resource, Guangxi Normal University, Guilin, China
,
Jiang Zhi
1   Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China
2   College of Environment and Resource, Guangxi Normal University, Guilin, China
› Author Affiliations
Supported by: National Natural Science Foundation of China 41661077

Abstract

Endophytic fungi can produce beneficial active components during symbiosis with host plants. Fifteen purified endophytic fungal strains were isolated and screened from root, stem, leaf, and fruit of the Chinese medicine food homologous plant Siraitia grosvenorii. These strains were fermented, and then the fermented solution was extracted using acetonitrile. The extracts were analyzed by high performance liquid chromatography (HPLC) and mass spectrum (MS), using standard mogroside V as control. The results showed that strain LHG-F5 and strain LHG-L4 could produce mogroside V with output of 3.814 µg/mL and 2.675 µg/mL, respectively. By morphological observation together with similarity of internal transcribed spacer (ITS) sequence by Clustan X method, LHG-F5 and LHG-L4 were classified as Diaporthe angelica and Fusarium solani, respectively.



Publication History

Received: 01 July 2019

Accepted after revision: 18 January 2020

Article published online:
31 January 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Stuttgart · New York

 
  • References

  • 1 Matsumoto K, Kasai R, Ohtani K, Tanaka O. Minor cucurbitane glycosides from fruits of Siraitia grosvenori (Cucurbitaceae). Chem Pharm Bull 1990; 38: 2030-2032
  • 2 Li DP, Zhang HR. Studies and uses of Chinese medicine Monkʼs Fruit – a special local product of Guangxi province, China. Guihaia 2000; 20: 270-276
  • 3 The Pharmacopoeia Commission of the Ministry of Public Health. The Pharmacopoeia of the Peopleʼs Republic of China 2015 Edition. Beijing: China Medical Science Press; 2015: 212
  • 4 Takemoto T, Arihara S, Nakajima T, Okuhira M. Studies on the constituents of Fructus Momordicae. I. On the sweet principle. Yakugaku Zasshi 1983; 103: 1151-1154
  • 5 Takemoto T, Arihara S, Nakajima T, Okuhira M. Studies on the constituents of Fructus Momordicae. II. Structure of sapogenin. Yakugaku Zasshi 1983; 103: 1155-1166
  • 6 Takemoto T, Arihara S, Nakajima T, Okuhira M. Studies on the constituents of Fructus Momordicae. III. Structure of mogrosides. Yakugaku Zasshi 1983; 103: 1167-1173
  • 7 Pawar RS, Krynitsky AJ, Rader JI. Sweeteners from plants – with emphasis on Stevia rebaudiana (Bertoni) and Siraitia grosvenorii (Swingle). Anal Bioanal Chem 2013; 405: 4397-4407
  • 8 Jia Z, Yang X. A minor, sweet cucurbitane from Siraitia grosvenorii . Nat Prod Commun 2010; 4: 769-772
  • 9 Zheng YB, Yu J, Liu JM. Determination of total saponin content in the fresh fruit of Siraitia grosvenorii. Annual Meeting Proceedings of Sweetener Professional Board, Production and Application of Food Additives Industry Associate, 2007.
  • 10 Li HB, Zhang M, Wang Y. Colorimetric determination of triterpenoid saponin in Luohanguo. Food Sci 2006; 27: 171-173
  • 11 Li C, Lin LM, Sui F, Wang ZM, Huo HR, Dai L, Jiang TL. Chemistry and pharmacology of Siraitia grosvenorii: a review. Chin J Nat Med 2014; 12: 89-102
  • 12 Petrini O, Fisher PJ. Fungal endophytes in Salicornia perennis . Trans British Mycol Soc 1986; 87: 647-651
  • 13 Huang WY, Cai YZ, Xing J, Corke H, Sun M. A potential antioxidant resource: endophytic fungi from medicinal plants. Econ Bot 2007; 61: 14-30
  • 14 Staniek A, Woerdenbag HJ, Kayser O. Endophytes: exploiting biodiversity for the improvement of natural product-based drug discovery. J Plant Int 2008; 3: 75-93
  • 15 Higginbotham SJ, Arnold AE, Ibañez A, Spadafora C, Coley PD, Kursar TA. Bioactivity of fungal endophytes as a function of endophyte taxonomy and the taxonomy and distribution of their host plants. PLoS One 2013; 8: e73192
  • 16 Priti V, Ramesha BT, Singh S, Avikanth G, Ganeshaiah KN, Suryanarayanan TS, Shaanker RU. How promising are endophytic fungi as alternative sources of plant secondary metabolites?. Curr Sci India 2009; 97: 477-478
  • 17 Liu JL, Li DP, Huang YL. Determination of mogrol glycocides and mogroside V content in the fresh fruit of some Siraitia grosvenorii varieties from different places in North Guangxi. Guihaia 2007; 27: 281-284