Xanthomonas phage RiverRider is a novel N4-like bacteriophage and the first phage isolated from the plant pathogen Xanthomonas fragariae. Electron microscopy revealed a Podoviridae morphology consisting of isometric heads and short noncontractile tails. The complete genome of RiverRider is 76,355 bp in length, with 90 open reading frames and seven tRNAs. The genome is characteristic of N4-like bacteriophages in both content and organization, having predicted proteins characterized into the functional groups of transcription, DNA metabolism, DNA replication, lysis, lysis inhibition, structure and DNA packaging. Amino acid sequence comparisons for proteins in these categories showed highest similarities to well-characterized N4-like bacteriophages isolated from Achromobacter xylosoxidans and Erwinia amylovora. However, the tail fiber proteins of RiverRider are clearly distinct from those of other N4-like phages. RiverRider was able to infect seven different strains of X. fragariae and none of the other species of Xanthomonas tested.

中文翻译：

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Xanthomonas噬菌体RiverRider的完整基因组序列，它是一种新型的N4样噬菌体，可感染草莓病原体Xanthomonas fragariae。

Xanthomonas噬菌体RiverRider是一种新型的N4样噬菌体，是从植物病原体Xanthomonas fragariae分离出的第一个噬菌体。电子显微镜检查显示了由等长头和短而无收缩尾巴组成的弓形病毒科形态。RiverRider的完整基因组长度为76,355 bp，具有90个开放阅读框和7个tRNA。基因组在内容和组织上都是N4样噬菌体的特征，具有预测的蛋白质，其特征在于转录，DNA代谢，DNA复制，裂解，裂解抑制，结构和DNA包装等功能组。这些类别蛋白质的氨基酸序列比较显示，与从木糖氧化无色杆菌和解淀粉欧文氏菌中分离出的特征明确的N4样噬菌体具有最高的相似性。然而，RiverRider的尾纤维蛋白明显不同于其他N4样噬菌体。RiverRider能够感染7种不同的草莓角斑病菌菌株，而其他测试的Xanthomonas菌株均未感染。