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Complete genome sequence of Xanthomonas phage RiverRider, a novel N4-like bacteriophage that infects the strawberry pathogen Xanthomonas fragariae

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Abstract

Xanthomonas phage RiverRider is a novel N4-like bacteriophage and the first phage isolated from the plant pathogen Xanthomonas fragariae. Electron microscopy revealed a Podoviridae morphology consisting of isometric heads and short noncontractile tails. The complete genome of RiverRider is 76,355 bp in length, with 90 open reading frames and seven tRNAs. The genome is characteristic of N4-like bacteriophages in both content and organization, having predicted proteins characterized into the functional groups of transcription, DNA metabolism, DNA replication, lysis, lysis inhibition, structure and DNA packaging. Amino acid sequence comparisons for proteins in these categories showed highest similarities to well-characterized N4-like bacteriophages isolated from Achromobacter xylosoxidans and Erwinia amylovora. However, the tail fiber proteins of RiverRider are clearly distinct from those of other N4-like phages. RiverRider was able to infect seven different strains of X. fragariae and none of the other species of Xanthomonas tested.

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Funding

Financial support was provided in part by The Banack Family Partnership Endowed Teaching Chair in Agriculture, to T.D.

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Authors and Affiliations

  1. Lloyd L. Gregory School of Pharmacy, Palm Beach Atlantic University, 901 S. Flagler Drive, P.O. Box 24708, West Palm Beach, FL, 33416, USA

    Morgan Miller

  2. UF Interdisciplinary Center for Biotechnology Research, Gene Expression and Genotyping Core, 2033 Mowry Road, Gainesville, FL, 32610, USA

    Alex Deiulio

  3. Biology Department, Indian River State College, 3209 Virginia Ave., Fort Pierce, FL, 34981, USA

    Chris Holland, Cayce Douthitt, Jessica McMahon, Helen Wiersma-Koch & Tom D’Elia

  4. USDA, ARS, U.S. Horticultural Research Laboratory, 2001 South Rock Rd, Fort Pierce, FL, 34945, USA

    William W. Turechek

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  1. Morgan Miller
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  4. Cayce Douthitt
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Correspondence to Tom D’Elia.

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Miller, M., Deiulio, A., Holland, C. et al. Complete genome sequence of Xanthomonas phage RiverRider, a novel N4-like bacteriophage that infects the strawberry pathogen Xanthomonas fragariae. Arch Virol 165, 1481–1484 (2020). https://doi.org/10.1007/s00705-020-04614-6

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  • DOI: https://doi.org/10.1007/s00705-020-04614-6

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