With the rapid depletion of crude resources, microorganism lipids have started attracting increasing attention because of their renewable qualities. However, their production is limited by high costs. In this study, we aimed to reduce the production cost of Sporidiobolus pararoseus JD-2 lipid by co-producing extracellular polysaccharide (EPS) and single-cell protein (SCP). In batch fermentation, the yields of lipid, EPS and SCP under ammonia nitrogen limitation increased by 20.3%, 32.0% and 43.7%, respectively, compared with the yields in the control group (without NH₄⁺). Next, fed-batch fermentation was performed under different ammonia nitrogen levels. The yield, productivity and coefficient of lipid reached 47.1 ± 1.1 g/L, 0.66 g/L/h and 0.250 g/g, respectively, under an ammonia nitrogen level of 20 g/L (NH₄)₂SO₄. In the same process, 14.3 ± 1.6 g/L EPS and 12.7 ± 0.8 g/L SCP were also obtained. Nutrient analysis of the product revealed that NH₄⁺ affected the proportion of pigments in the carotenoids and increased the content of unsaturated fatty acids in the lipid; EPS mainly comprised galactose, glucose, mannose and fucose, at a ratio of approximately 45:37:2:1; and the essential amino acid content in SCP accounted for 48% of the product. Thus, this study provided a new strategy for improving S. pararoseus JD-2 lipid production at a lower cost.

随着原油资源的迅速消耗，微生物脂质由于其可再生的特性而开始引起越来越多的关注。但是，它们的生产受到高成本的限制。在这项研究中，我们旨在通过共同生产细胞外多糖（EPS）和单细胞蛋白（SCP）来降低副糖螺旋体JD-2脂质的生产成本。在分批发酵中，与不添加NH ₄ ⁺的对照组相比，在氨氮限制下的脂质，EPS和SCP的产量分别增加了20.3％，32.0％和43.7％。）。接下来，在不同的氨氮水平下进行分批补料发酵。在20 g / L（NH ₄）₂ SO ₄的氨氮水平下，脂质的产量，生产率和系数分别达到47.1±1.1 g / L，0.66 g / L / h和0.250 g / g 。在相同的过程中，还获得了14.3±1.6 g / L EPS和12.7±0.8 g / L SCP。产品的营养分析表明，NH ₄ ⁺影响类胡萝卜素中色素的比例并增加脂质中不饱和脂肪酸的含量；EPS主要包含半乳糖，葡萄糖，甘露糖和岩藻糖，比例约为45：37：2：1；SCP中的必需氨基酸含量占该产品的48％。因此，这项研究提供了一种以较低的成本改善副玫瑰糖链球菌JD-2脂质生产的新策略。