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Co-production of lipid, exopolysaccharide and single-cell protein by Sporidiobolus pararoseus under ammonia nitrogen-limited conditions

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Abstract

With the rapid depletion of crude resources, microorganism lipids have started attracting increasing attention because of their renewable qualities. However, their production is limited by high costs. In this study, we aimed to reduce the production cost of Sporidiobolus pararoseus JD-2 lipid by co-producing extracellular polysaccharide (EPS) and single-cell protein (SCP). In batch fermentation, the yields of lipid, EPS and SCP under ammonia nitrogen limitation increased by 20.3%, 32.0% and 43.7%, respectively, compared with the yields in the control group (without NH4+). Next, fed-batch fermentation was performed under different ammonia nitrogen levels. The yield, productivity and coefficient of lipid reached 47.1 ± 1.1 g/L, 0.66 g/L/h and 0.250 g/g, respectively, under an ammonia nitrogen level of 20 g/L (NH4)2SO4. In the same process, 14.3 ± 1.6 g/L EPS and 12.7 ± 0.8 g/L SCP were also obtained. Nutrient analysis of the product revealed that NH4+ affected the proportion of pigments in the carotenoids and increased the content of unsaturated fatty acids in the lipid; EPS mainly comprised galactose, glucose, mannose and fucose, at a ratio of approximately 45:37:2:1; and the essential amino acid content in SCP accounted for 48% of the product. Thus, this study provided a new strategy for improving S. pararoseus JD-2 lipid production at a lower cost.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Number 31601459); Science and Technology support Program of Jiangsu Province (BE2018316); Top-Notch Academic Programs Project of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Hongtao Wang, Bin Hu, Jie Liu, Jianzhong Xu & Weiguo Zhang

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    He Qian

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  1. Hongtao Wang
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  2. Bin Hu
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Wang, H., Hu, B., Liu, J. et al. Co-production of lipid, exopolysaccharide and single-cell protein by Sporidiobolus pararoseus under ammonia nitrogen-limited conditions. Bioprocess Biosyst Eng 43, 1403–1414 (2020). https://doi.org/10.1007/s00449-020-02335-3

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