Laxaphycins B5 and B6 from the cultured cyanobacterium UIC 10484.,The Journal of Antibiotics

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Laxaphycins B5 and B6 from the cultured cyanobacterium UIC 10484.
The Journal of Antibiotics ( IF 2.1 ) Pub Date : 2020-03-31 , DOI: 10.1038/s41429-020-0301-x
Peter Sullivan ₁ , Aleksej Krunic ₁ , Joanna E Burdette ₁ , Jimmy Orjala ₁

Affiliation

Two laxaphycin type-B cyclic dodecapeptides, laxaphycins B5 and B6, were obtained from UIC 10484, a freshwater cf. Phormidium sp. Analysis using the 16S rRNA sequence found UIC 10484 to clade with UIC 10045, a known laxaphycin type-A and -B producer, and MS/MS analysis revealed the presence of two novel laxaphycin type-B compounds. The structures of the metabolites were elucidated using 2D NMR and MS/MS. The absolute configurations of the amino acids were determined by advanced Marfey's analysis. Both metabolites were evaluated against the same three cancer cell lines. The IC50 of both laxaphycins B5 and B6 was near 1 μM against breast cancer MDA-MB-231, melanoma MDA-MB-435, and ovarian cancer OVCAR3 cell lines.

中文翻译：

来自培养的蓝细菌 UIC 10484 的 Laxaphycins B5 和 B6。

两种 laxaphycin B 型环状十二肽，laxaphycins B5 和 B6，从 UIC 10484 获得，参见淡水。Phormidium sp. 使用 16S rRNA 序列进行的分析发现 UIC 10484 与 UIC 10045（一种已知的拉克霉素 A 型和 B 型生产者）进化枝，MS/MS 分析显示存在两种新型拉克霉素 B 型化合物。使用 2D NMR 和 MS/MS 阐明了代谢物的结构。氨基酸的绝对构型由高级 Marfey 分析确定。两种代谢物均针对相同的三种癌细胞系进行评估。Laxaphycins B5 和 B6 对乳腺癌 MDA-MB-231、黑色素瘤 MDA-MB-435 和卵巢癌 OVCAR3 细胞系的 IC50 接近 1 μM。

更新日期：2020-03-31

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