Abstract

The marked heterogeneity of autism spectrum disorders is a challenge for research into biomarkers of these disorders. The present study aimed to assess the association of the thioredoxin (TRX) system and vasopressin with autism in children. Thirty-five autistic children were recruited with thirty-one healthy controls children matched for age and gender. Total antioxidant capacity (TAC), vasopressin, peroxiredoxin (PRDX1, PRDX3), phox domain-containing protein kinase-like protein, and TRX1 levels were measured in plasma and mercury and lead levels were measured in red blood cells. Children with autism had significantly lower levels of PRDX1 and TRX1 and significantly higher levels of lead and mercury compared with controls. Significantly higher levels of vasopressin were also observed in more severe cases of autism. Receiver operating characteristics analysis demonstrated satisfactory specificity and sensitivity of TAC, PRDX1, PRDX3, and TRX1 for the diagnosis of autism. The optimal cut-off value for plasma TRX1 levels, as an indicator of autism, was 10.6 ng/mL. Significant positive correlations existed between TRX system components, lead, and mercury. A negative correlation was found between vasopressin and TRX1, but positive correlations were found between vasopressin and mercury and lead. In conclusion, TAC, PRDX1, PRDX3, and TRX1 may be useful biomarkers for the diagnosis of autism in Saudi populations. Vasopressin-resistance in autistic patients may be due to an increased oxidative burden.

中文翻译：

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加压素与某些氧化应激标记物有关的自闭症病因机制

摘要

自闭症谱系障碍的明显异质性是对这些障碍的生物标志物进行研究的挑战。本研究旨在评估硫氧还蛋白（TRX）系统和加压素与儿童自闭症的关系。招募了35名自闭症儿童和31名年龄和性别相匹配的健康对照儿童。测定血浆中的总抗氧化剂容量（TAC），血管加压素，过氧化物酶（PRDX1，PRDX3），含phox结构域的蛋白激酶样蛋白和TRX1的水平，并测量红血球中的汞和铅的水平。与对照组相比，自闭症儿童的PRDX1和TRX1含量明显降低，铅和汞的含量明显升高。在更严重的自闭症患者中也观察到了较高的血管加压素水平。接受者的工作特征分析表明，TAC，PRDX1，PRDX3和TRX1对自闭症的诊断具有令人满意的特异性和敏感性。作为自闭症指标的血浆TRX1水平的最佳临界值为10.6 ng / mL。TRX系统组分，铅和汞之间存在显着的正相关关系。血管加压素与TRX1之间呈负相关，而血管加压素与汞和铅之间呈正相关。总之，TAC，PRDX1，PRDX3和TRX1可能是诊断沙特人群自闭症的有用生物标记。自闭症患者对血管加压素的抵抗可能是由于增加的氧化负荷所致。作为自闭症指标的血浆TRX1水平的最佳临界值为10.6 ng / mL。TRX系统组分，铅和汞之间存在显着的正相关关系。血管加压素与TRX1之间呈负相关，而血管加压素与汞和铅之间呈正相关。总之，TAC，PRDX1，PRDX3和TRX1可能是诊断沙特人群自闭症的有用生物标记。自闭症患者对血管加压素的抵抗可能是由于增加的氧化负荷所致。作为自闭症指标的血浆TRX1水平的最佳临界值为10.6 ng / mL。TRX系统组分，铅和汞之间存在显着的正相关关系。血管加压素与TRX1之间呈负相关，而血管加压素与汞和铅之间呈正相关。总之，TAC，PRDX1，PRDX3和TRX1可能是诊断沙特人群自闭症的有用生物标记。自闭症患者对血管加压素的抵抗可能是由于增加的氧化负荷所致。TRX1和TRX1可能是诊断沙特人群自闭症的有用生物标志物。自闭症患者对血管加压素的抵抗可能是由于增加的氧化负荷所致。TRX1和TRX1可能是诊断沙特人群自闭症的有用生物标志物。自闭症患者对血管加压素的抵抗可能是由于增加的氧化负荷所致。