Abstract

The inhibitory effect of mercury(II) chloride on the activity and structure of the hydrogenase Thiocapsa roseopersicina BBS was studied. The kinetics of hydrogenase inactivation in the presence of different inhibitor concentrations was determined. The irreversible nature of Hg²⁺ action was established, and hydrogenase inhibition constants at different temperatures were determined. The presence of this inhibitor in enzyme solution significantly reduced its stability and caused denaturation at temperatures above 50°C. In the process of enzyme incubation with Hg²⁺, the absorption band fades in the visible region of the spectrum, indicating the destruction of iron–sulfur clusters. Comparative analysis of the infrared Fourier spectra of hydrogenase without the addition and after incubation with inhibitor indicates the destruction of the NiFe active center.

中文翻译：

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Hg 2+对紫色硫细菌Thiocapsa roseopersicina BBS的HydSL氢化酶的影响

摘要

研究了氯化汞（II）对加氢玫瑰硫硫杆菌BBS活性和结构的抑制作用。确定了在不同抑制剂浓度下氢化酶失活的动力学。建立了Hg ²⁺作用的不可逆性质，并确定了不同温度下的氢化酶抑制常数。酶溶液中这种抑制剂的存在会大大降低其稳定性，并在高于50°C的温度下引起变性。在Hg ²⁺孵育酶的过程中，吸收带在光谱的可见光区域消失，表明铁硫簇的破坏。对未添加和与抑制剂孵育后的氢化酶的红外傅里叶光谱进行的比较分析表明，NiFe活性中心被破坏。