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Effect of Hg2+ on HydSL Hydrogenase of the Purple Sulfur Bacteria Thiocapsa roseopersicina BBS

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Abstract

The inhibitory effect of mercury(II) chloride on the activity and structure of the hydrogenase Thiocapsa roseopersicina BBS was studied. The kinetics of hydrogenase inactivation in the presence of different inhibitor concentrations was determined. The irreversible nature of Hg2+ action was established, and hydrogenase inhibition constants at different temperatures were determined. The presence of this inhibitor in enzyme solution significantly reduced its stability and caused denaturation at temperatures above 50°C. In the process of enzyme incubation with Hg2+, the absorption band fades in the visible region of the spectrum, indicating the destruction of iron–sulfur clusters. Comparative analysis of the infrared Fourier spectra of hydrogenase without the addition and after incubation with inhibitor indicates the destruction of the NiFe active center.

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ACKNOWLEDGMENTS

The authors are grateful to A.A. Zabelin for assistance in the recording and processing of the IR Fourier spectra of hydrogenase.

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Separate Division of the Federal State Budgetary Institution of Science Federal Research Center, Pushchino Research Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    N. A. Zorin, A. S. Starodubov & A. A. Tsygankov

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  1. N. A. Zorin
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  2. A. S. Starodubov
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  3. A. A. Tsygankov
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Correspondence to N. A. Zorin.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Zorin, N.A., Starodubov, A.S. & Tsygankov, A.A. Effect of Hg2+ on HydSL Hydrogenase of the Purple Sulfur Bacteria Thiocapsa roseopersicina BBS. Appl Biochem Microbiol 56, 149–153 (2020). https://doi.org/10.1134/S0003683820020155

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