This article presents 20 combinations of histochemical stainings for the determination of mast cell co-localization with the fibrous component of the connective tissue in the fibrillogenesis course. Best results were obtained using metachromatic detection of mast cells in combination with silver or picro-fuchsin impregnation, staining with brilliant green using van Gieson staining, and a combination of aniline blue staining with neutral red. Proposed variants of histochemical protocols open up new opportunities to analyze the participation of mast cells in extracellular matrix remodeling of the tissue microenvironment in the course of adaptive and pathological processes. Results obtained expand the current theoretical views of the process of fibrillogenesis in the extracellular matrix. They also shed new light on the participation of mast cell secretion components in the molecular mechanisms of fiber formation.

本文介绍了20种组织化学染色组合，用于确定在原纤维形成过程中肥大细胞与结缔组织纤维成分的共定位。肥大细胞的变色检测结合银或微红红霉素的浸渍，van Gieson染色用亮绿色染色，以及苯胺蓝染色和中性红结合使用可获得最佳结果。组织化学方案的拟议变体为分析肥大细胞在适应性和病理过程中参与组织微环境的细胞外基质重塑提供了新的机会。获得的结果扩展了细胞外基质中原纤维形成过程的当前理论观点。