Tospoviruses cause significant losses to a wide range of agronomic and horticultural crops worldwide. The type member, Tomato spotted wilt tospovirus (TSWV), causes systemic infection in susceptible tomato cultivars, whereas its infection is localized in cultivars carrying the Sw-5 resistance gene. The response to TSWV infection in tomato cultivars with or without Sw-5 was determined at the virus small RNA level in the locally infected leaf. Predicted reads were aligned to TSWV reference sequences. The TSWV genome was found to be differentially processed among each of the three-viral genomic RNAs-Large (L), Medium (M) and Small (S)-in the Sw-5(+) compared to Sw-5(-) genotypes. In the Sw-5(+) cultivar, the L RNA had the highest number of viral small-interfering RNAs (vsiRNAs), whereas in the Sw-5(-) cultivar the number was higher in the S RNA. Among the three-viral genomic RNAs, the distribution of hotspots showed a higher number of reads per million reads of vsiRNAs of 21 and 22 nt class at the 5' and 3' ends of the L and the S RNAs, with less coverage in the M RNA. In the Sw-5(-) cultivar, the nature of the 5' nucleotide-end in the siRNAs varied significantly; reads with 5'-adenine-end were most abundant in the mock control, whereas cytosine and uracil were more abundant in the infected plants. No such differences were seen in case of the resistant genotype. Findings provided insights into the response of tomato cultivars to TSWV infection.

中文翻译：

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带有或不带有Sw-5基因的TSWV感染番茄（Solanum lycopersicum）均以番茄斑点枯萎病毒（TSWV）基因组为目标。

脊灰病毒对全世界范围广泛的农艺和园艺作物造成重大损失。类型成员“番茄斑点枯萎病毒”（TSWV）在易感番茄品种中引起系统感染，而其感染局限于携带Sw-5抗性基因的品种。在有或没有Sw-5的番茄品种中，对TSWV感染的反应是通过局部感染叶片中的病毒小RNA水平确定的。将预测的读数与TSWV参考序列比对。发现TSWV基因组与Sw-5（-）相比，在Sw-5（+）中三个病毒基因组RNA的大（L），中（M）和小（S）-的差异处理。基因型。在Sw-5（+）品种中，L RNA的病毒小干扰RNA（vsiRNA）数量最多，而在Sw-5（-）品种中，S RNA的数量更高。在三病毒基因组RNA中，热点分布表明，在L和S RNA的5'和3'末端，每百万读入21和22 nt类的vsiRNA的读码数量更高。 M RNA。在Sw-5（-）品种中，siRNA的5'核苷酸末端的性质差异很大。在模拟对照中，具有5'-腺嘌呤末端的读段最丰富，而在被感染的植物中胞嘧啶和尿嘧啶更丰富。在抗性基因型的情况下，未见此类差异。这些发现为番茄品种对TSWV感染的反应提供了见解。在Sw-5（-）品种中，siRNA的5'核苷酸末端的性质差异很大。在模拟对照中，具有5'-腺嘌呤末端的读段最丰富，而在被感染的植物中胞嘧啶和尿嘧啶更丰富。在抗性基因型的情况下，未见此类差异。这些发现为番茄品种对TSWV感染的反应提供了见解。在Sw-5（-）品种中，siRNA的5'核苷酸末端的性质差异很大。在模拟对照中，具有5'-腺嘌呤末端的读段最丰富，而在被感染的植物中胞嘧啶和尿嘧啶更丰富。在抗性基因型的情况下，未见此类差异。这些发现为番茄品种对TSWV感染的反应提供了见解。