Abstract

This study focuses on the synthesis and characterization of Ag nanoparticle-decorated reduced graphene oxide (RGO) nanocomposites (Ag-RGO), which were obtained via a facile, green and efficient one-pot hydrothermal method. TEM results showed that the silver nanoparticles (20-50 nm) were uniformly attached onto RGO sheets with C/O ratio of 6.4 and 80% of sp² carbon, obtained from XPS analysis. Compared with GO and RGO, the prepared Ag-RGO composites possessed significant antibacterial property; the minimum inhibitory concentration of Ag-RGO was just 31.25 μg/mL against Gram-positive bacteria S. aureus and Gram-negative bacteria E. coli. Particularly, the Ag-RGO nanocomposites released more Ag⁺ in acidic condition, promoting the produce of reactive oxygen species, which are acknowledged as disruption to the membrane integrity of the bacteria. Moreover, the Ag-RGO nanocomposites effectively dispersed and eliminated the maturely formed biofilm, showing superior advantages in responding to public health threats posed by biofilm-related infections.

中文翻译：

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一步法合成Ag还原抗氧化膜的氧化石墨烯纳米复合材料

摘要

这项研究的重点是合成和表征的银纳米粒子修饰的还原氧化石墨烯（RGO）纳米复合材料（Ag-RGO），这是通过一种简便，绿色，高效的一锅水热法获得的。TEM结果表明，银纳米颗粒（20-50 nm）均匀附着在RGO片材上，C / O值为6.4，80％的sp ²碳来自XPS分析。与GO和RGO相比，制备的Ag-RGO复合材料具有明显的抗菌性能。Ag-RGO对革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌大肠杆菌的最小抑菌浓度仅为31.25μg/ mL 。特别是，Ag-RGO纳米复合材料释放出更多的Ag ⁺在酸性条件下，可促进活性氧的产生，这被认为是破坏细菌膜完整性的原因。而且，Ag-RGO纳米复合材料有效地分散并消除了成熟形成的生物膜，在应对生物膜相关感染所构成的公共卫生威胁方面显示出优越的优势。