The present study aimed to estimate the effects of high-protein diet (PD)-isolated whey protein and omega-3 fatty acids-docosahexaenoic and eicosapentaenoic acid on oxidative parameters of rats treated with Olanzapine (OLZ). Experiments were carried out on 8-week-old Wistar albino male rats (n = 64) weighing 200 ± 20 g. By dietary and pharmacological treatment, all animals were divided into 8 groups: 1. CTRL group; 2. CTRL + OLZ group; 3. CTRL + FA group; 4. CTRL + OLZ + FA group; 5. PD group; 6. PD + OLZ group; 7. PD + FA group; 8. PD + OLZ + FA group. After 6 weeks of pharmacological/diet treatment, all animals were sacrificed to collect blood samples and determine the biomarkers of oxidative stress. The following oxidative stress markers were measured spectrophotometrically: superoxide anion radical (O2-), hydrogen peroxide (H2O2), nitric oxide (NO-), index of lipid peroxidation measured as TBARS, reduced glutathione, catalase and superoxide dismutase. The study has shown that Olanzapine treatment was associated with increased release of pro-oxidants and diminished activity of anti-oxidant markers. Additional supplementation with PD and FA succeeded in abolishing the negative influence in most of the measured parameters. However, these beneficial impacts were stronger in the case of their separate application, which could be the practical and clinical importance of these results.

中文翻译：

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高蛋白饮食和omega-3脂肪酸可改善奥氮平治疗大鼠的氧化还原状态。

本研究旨在评估高蛋白饮食（PD）分离的乳清蛋白和omega-3脂肪酸-二十二碳六烯酸和二十碳五烯酸对奥氮平（OLZ）治疗的大鼠氧化参数的影响。实验在体重200±20 g的8周大的Wistar白化雄性大鼠（n = 64）上进行。通过饮食和药物治疗，将所有动物分为8组：1.CTRL组； 2。对照组。2. CTRL + OLZ组；3. CTRL + FA组；4. CTRL + OLZ + FA组；5. PD组；6. PD + OLZ组；7. PD + FA组；8. PD + OLZ + FA组。药物/饮食治疗6周后，处死所有动物以收集血样并确定氧化应激的生物标记。分光光度法测量了以下氧化应激标记：超氧阴离子自由基（O2-），过氧化氢（H2O2），一氧化氮（NO-），脂质过氧化指数，以TBARS表示，还原型谷胱甘肽，过氧化氢酶和超氧化物歧化酶。研究表明，奥氮平治疗与促氧化剂释放增加和抗氧化剂标志物活性降低有关。PD和FA的额外补充成功消除了大多数测量参数中的负面影响。但是，这些有益效果在单独应用的情况下更强，这可能是这些结果的实践和临床重要性。PD和FA的额外补充成功消除了大多数测量参数中的负面影响。但是，这些有益效果在单独应用的情况下更强，这可能是这些结果的实践和临床重要性。PD和FA的额外补充成功消除了大多数测量参数中的负面影响。但是，这些有益效果在单独应用的情况下更强，这可能是这些结果的实践和临床重要性。