Abstract
The present study aimed to estimate the effects of high-protein diet (PD)—isolated whey protein and omega-3 fatty acids—docosahexaenoic and eicosapentaenoic acid on oxidative parameters of rats treated with Olanzapine (OLZ). Experiments were carried out on 8-week-old Wistar albino male rats (n = 64) weighing 200 ± 20 g. By dietary and pharmacological treatment, all animals were divided into 8 groups: 1. CTRL group; 2. CTRL + OLZ group; 3. CTRL + FA group; 4. CTRL + OLZ + FA group; 5. PD group; 6. PD + OLZ group; 7. PD + FA group; 8. PD + OLZ + FA group. After 6 weeks of pharmacological/diet treatment, all animals were sacrificed to collect blood samples and determine the biomarkers of oxidative stress. The following oxidative stress markers were measured spectrophotometrically: superoxide anion radical (O2−), hydrogen peroxide (H2O2), nitric oxide (NO−), index of lipid peroxidation measured as TBARS, reduced glutathione, catalase and superoxide dismutase. The study has shown that Olanzapine treatment was associated with increased release of pro-oxidants and diminished activity of anti-oxidant markers. Additional supplementation with PD and FA succeeded in abolishing the negative influence in most of the measured parameters. However, these beneficial impacts were stronger in the case of their separate application, which could be the practical and clinical importance of these results.
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Acknowledgements
This work was supported by Junior projects 01/15 and 03/18 of Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
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Conceptualization: MM, VLJ, Sergey Bolevich; Methodology: VZ, IS, TNT, AV, ZP, JS; Writing—review and editing: VZ, TNT; Supervision: MM, VLJ, Sergey Bolevich. All authors have read the manuscript and authorized the submission for publication.
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This study was conducted in the laboratory for Cardiovascular Physiology (Faculty of Medical Sciences, University of Kragujevac, Serbia). The study protocol was approved by the Ethical Committee for the welfare of experimental animals of the Faculty of Medical Sciences, University of Kragujevac, Serbia (permit number: 01-12860). All experiments were performed according to EU Directive for welfare of laboratory animals (86/609/EEC) and principles of Good Laboratory Practice.
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Mitrovic, M., Nikolic Turnic, T., Zivkovic, V. et al. High-protein diet and omega-3 fatty acids improve redox status in olanzapine-treated rats. Mol Cell Biochem 468, 143–152 (2020). https://doi.org/10.1007/s11010-020-03718-6
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DOI: https://doi.org/10.1007/s11010-020-03718-6