A systematic adaptive laboratory evolution strategy was employed to develop a potential Zymomonas mobilis strain with the ability to co-utilize glucose and xylose. Z. mobilis ATCC ZW658, a recombinant xylose fermenting strain, was subjected to adaptive laboratory evolution over a period of 200 days under strict selection pressure of increasing concentration of xylose. The evolved strain exhibited 1.65 times increase in the overall specific xylose utilization rate when compared with the parent strain. Furthermore, the strain displayed significantly improved performance in terms of co-fermentation of xylose in the presence of glucose with specific glucose and xylose utilization rate of 1.24 g g⁻¹ h⁻¹ and 1.34 g g⁻¹ h⁻¹, respectively. Altered phenotypic response of the evolved strain, in terms of improved xylose utilization, co-utilization of mixed sugars, enhanced growth, ethanol production, and reduced xylitol production has been explained by novel mutations, identified using next-generation sequencing, in xylose assimilating, metabolizing, and crucial regulatory pathway genes and key enzyme activity assays.

一种系统的适应性实验室进化策略被用来开发潜在的运动发酵单胞菌菌株，具有共同利用葡萄糖和木糖的能力。运动发酵单胞菌ATCC ZW658，一种重组木糖发酵菌株，在增加木糖浓度的严格选择压力下，经过200天的适应性实验室进化。与亲本菌株相比，进化菌株显示出总的特定木糖利用率提高了1.65倍。此外，该菌株在葡萄糖存在下与木糖共发酵方面表现出显着改善的性能，比葡萄糖和木糖利用率分别为1.24 g g ^-1 h ^-1和1.34 g g ^-1h ^-1。在改进的木糖利用，混合糖的共同利用，增长的增长，乙醇生产和木糖醇生产减少方面，进化菌株的表型反应发生了变化，这可以通过使用新一代测序技术鉴定出的新突变进行解释，这些突变是木糖同化，代谢和关键的调控途径基因以及关键的酶活性测定。