A microfluidic co-culture system, consisting of mouse embryonic stem cells (mESCs)/OP9 cells, was evaluated as a platform for studying hematopoietic differentiation mechanisms in vitro. mESC differentiation into blood cells was achieved in a microchannel that had the minimum size necessary to culture cells. The number of generated blood cells increased or decreased based on the nitric oxide (NO) donor or inhibitor used. Conditioned medium from OP9 cell cultures also promoted an increase in the number of blood cells. The number of generated blood cells under normal medium flow conditions was lower than that observed under the static condition. However, when using a conditioned medium, the number of generated blood cells under flow conditions was the same as that observed under the static condition. We conclude that secreted molecules from OP9 cells have a large influence on the differentiation of mESCs into blood cells. This is the first report of a microfluidic mESC/OP9 co-culture system that can contribute to highly detailed hematopoietic research studies by mimicking the cellular environment.

中文翻译：

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一氧化氮和条件培养基影响微流小鼠胚胎干细胞/ OP9共培养系统中的造血发育。

评价了由小鼠胚胎干细胞（mESCs）/ OP9细胞组成的微流体共培养系统，作为研究体外造血分化机制的平台。在具有培养细胞所需的最小尺寸的微通道中实现了mESC向血细胞的分化。生成的血细胞数量基于使用的一氧化氮（NO）供体或抑制剂而增加或减少。来自OP9细胞培养的条件培养基也促进了血细胞数量的增加。在正常介质流动条件下产生的血细胞数量要少于在静态条件下观察到的数量。然而，当使用条件培养基时，在流动条件下产生的血细胞数量与在静态条件下观察到的数量相同。我们得出的结论是，OP9细胞分泌的分子对mESCs分化为血细胞有很大影响。这是微流体mESC / OP9共培养系统的首次报道，该系统可通过模仿细胞环境为高度详细的造血研究做出贡献。