In order to quantitatively detect hydrogen polysulfides (H2Sn, n > 1) in biosystems, we propose a new two-photon turnon fluorescent probe, named N_p–S_n, based on the D–π–A skeleton two-photon fluorophore of 4 -hydroxy-1,8-naphthalimide derivative and deprotection of 2-fluoro-5-nitrobenzoic ester by H₂S_n–N_p–S_n displayed a more than 80-fold enhancement towards H2Sn in 550 nm and high sensitivity with a detection limit as low as 33 nM. Additionally, the probe N_p–S_n was further used for fluorescence imaging of H2Sn in living cells under two-photon excitation (820 nm), which showed a high-resolution imaging, thus demonstrating its practical application in biological systems for the study of physiological and pathological functions of H₂S_n.

中文翻译：

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基于4-羟基-1,8-萘二甲酰亚胺的开启式双光子荧光探针，用于多硫化氢感测

为了定量检测生物系统中的多硫化氢（H2Sn，n> 1），我们基于D–π–A骨架双光子荧光团4 ，提出了一种新的双光子开启荧光探针，名为N _p –S _n。 -羟基-1,8-萘二甲酰亚胺衍生物和H ₂ S _n –N _p –S _n对2-氟-5-硝基苯甲酸酯的脱保护显示，在550 nm下对H2Sn的增强作用超过80倍，并且具有高灵敏度，可检测到限制低至33 nM。此外，探针N _p –S _n进一步用于双光子激发（820 nm）下活细胞中H2Sn的荧光成像，显示了高分辨率的成像，从而证明了其在生物学系统中研究H ₂ S _n的生理和病理功能的实际应用。。