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A 4-Hydroxy-1,8-Naphthalimide-Based Turn-on Two-Photon Fluorescent Probe for Hydrogen Polysulfide Sensing
Journal of Applied Spectroscopy ( IF 0.8 ) Pub Date : 2020-01-21 , DOI: 10.1007/s10812-020-00942-2
L. Zhou , G. Yuan , Sh. Hu

In order to quantitatively detect hydrogen polysulfides (H2Sn, n > 1) in biosystems, we propose a new two-photon turnon fluorescent probe, named Np–Sn, based on the D–π–A skeleton two-photon fluorophore of 4 -hydroxy-1,8-naphthalimide derivative and deprotection of 2-fluoro-5-nitrobenzoic ester by H2Sn–Np–Sn displayed a more than 80-fold enhancement towards H2Sn in 550 nm and high sensitivity with a detection limit as low as 33 nM. Additionally, the probe Np–Sn was further used for fluorescence imaging of H2Sn in living cells under two-photon excitation (820 nm), which showed a high-resolution imaging, thus demonstrating its practical application in biological systems for the study of physiological and pathological functions of H2Sn.

中文翻译:

基于4-羟基-1,8-萘二甲酰亚胺的开启式双光子荧光探针,用于多硫化氢感测

为了定量检测生物系统中的多硫化氢(H2Sn,n> 1),我们基于D–π–A骨架双光子荧光团4 ,提出了一种新的双光子开启荧光探针,名为N p –S n。 -羟基-1,8-萘二甲酰亚胺衍生物和H 2 S n –N p –S n对2-氟-5-硝基苯甲酸酯的脱保护显示,在550 nm下对H2Sn的增强作用超过80倍,并且具有高灵敏度,可检测到限制低至33 nM。此外,探针N p –S n进一步用于双光子激发(820 nm)下活细胞中H2Sn的荧光成像,显示了高分辨率的成像,从而证明了其在生物学系统中研究H 2 S n的生理和病理功能的实际应用。。
更新日期:2020-01-21
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