Abstract

In the present work, the APX gene encoding ascorbate peroxidase in the moss Dicranum scoparium was for the first time cloned and sequenced, and a high homology of APX with ascorbate peroxidase genes of the mosses Grimmia pilifera and Physcomitrella patens was shown. The structure of the protein was characterized using bioinfomatics approach, and the activity of the enzyme under abiotic stresses was studied. An increase in the activity of ascorbate peroxidase was detected during desiccation of D. scoparium shoots. When exposed to heat shock, a decrease in the activity of ascorbate peroxidase correlated with a decrease in the expression of APX. Conserved elements, which were found in the structure of ascorbate peroxidase gene and protein, indicate that these sequences are conserved in the plant genome during evolution, in support of the importance of this enzyme in maintaining cellular redox status.

中文翻译：

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苔藓天牛抗坏血酸过氧化物酶：基因鉴定和酶活性。

摘要

在目前的工作中，APX编码抗坏血酸基因在苔藓过氧化物酶曲尾藓是首次克隆和测序，且具有很高的同源性APX与抗坏血酸过氧化物酶的基因苔藓紫萼pilifera和小立碗藓中所示。使用生物信息学方法表征了蛋白质的结构，并研究了在非生物胁迫下该酶的活性。D. scoparium芽干燥期间检测到抗坏血酸过氧化物酶活性的增加。当暴露于热激中时，抗坏血酸过氧化物酶活性的降低与APX表达的降低相关。在抗坏血酸过氧化物酶基因和蛋白质的结构中发现的保守元件，表明这些序列在进化过程中在植物基因组中是保守的，以支持该酶在维持细胞氧化还原状态中的重要性。