Abstract
In the present work, the APX gene encoding ascorbate peroxidase in the moss Dicranum scoparium was for the first time cloned and sequenced, and a high homology of APX with ascorbate peroxidase genes of the mosses Grimmia pilifera and Physcomitrella patens was shown. The structure of the protein was characterized using bioinfomatics approach, and the activity of the enzyme under abiotic stresses was studied. An increase in the activity of ascorbate peroxidase was detected during desiccation of D. scoparium shoots. When exposed to heat shock, a decrease in the activity of ascorbate peroxidase correlated with a decrease in the expression of APX. Conserved elements, which were found in the structure of ascorbate peroxidase gene and protein, indicate that these sequences are conserved in the plant genome during evolution, in support of the importance of this enzyme in maintaining cellular redox status.
Similar content being viewed by others
REFERENCES
Foyer, C.H., Environ. Exp. Bot., 2018, vol. 154, pp. 134–142. https://doi.org/10.1016/j.envexpbot.2018.05.003
Gest, N., Gautier, H., and Stevens, R., J. Exp. Bot., 2013, vol. 64, pp. 33–53. https://doi.org/10.1093/jxb/ers297
Smirnoff, N., Free Radic. Biol. Med., 2018, vol. 122, pp. 116–129. https://doi.org/10.1016/j.freeradbiomed.2018.03.033
Onele, A.O., Chasov, A., Viktorova, L., et al., S. Afr. J. Bot., 2018, vol. 119, pp. 132–141. https://doi.org/10.1016/j.sajb.2018.08.014
Li, X., Zhang, D., Li, H., et al., Front. Plant Sci., 2015, vol. 38, p. 6. https://doi.org/10.3389/fpls.2015.00038
Song, X.H., Sha, W., Jin, Z.M., et al., Adv. Intel. Soft. Compu., 2012, vol. 134, pp. 433–440. https://doi.org/10.1007/978-3-642-27537-1_53
Rushmore, T.H., Morton, M.R., and Pickett, C.B., J. Biol. Chem., 1991, vol. 266, pp. 11632–11639.
Dabrowska, G., Kata, A., Goc, A., et al., Acta Biol. Crac., 2007, vol. 1, pp. 7–17.
Jespersen, H.M., Kjaersgard, I.V.H., Ostergaard, L., et al., Biochem. J., 1997, vol. 326, pp. 305–310. https://doi.org/10.1042/bj3260305
Celik, A., Cullis, P.M., Sutcliffe, M.J., et al., Eur. J. Biochem., 2001, vol. 268, pp. 78–85. https://doi.org/10.1046/j.1432-1327.2001.01851.x
Bursey, E.H. and Poulos, T.L., Biochemistry, 2000, vol. 39, pp. 7374–737. https://doi.org/10.1021/bi000446s
Lubaina, A.S., Meenu Krishnan, V.G., and Murugan, K., IndianJ. Plant Sci., 2013, vol. 2, pp. 12–22.
Funding
This work was supported in part by the Russian Foundation for Basic Research (RFBR) (project no. 18-04-01060), joint grant of RFBR and Government of Republic of Tatarstan (project no. 18-44-160031), the programs for competitiveness of the Kazan Federal University, as well as within the framework of the State Assignment of the Kazan Research Center, Russian Academy of Sciences.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Additional information
Translated by M. Batrukova
Rights and permissions
About this article
Cite this article
Onele, A.O., Chasov, A.V., Trifonova, T.V. et al. Ascorbate Peroxidase of Moss Dicranum scoparium: Gene Identification and Enzyme Activity. Dokl Biochem Biophys 489, 380–384 (2019). https://doi.org/10.1134/S1607672919060085
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672919060085