Abstract

A method has been developed for the purification of a protease activator of protein C in human blood plasma from the culture fluid of micromycete Aspergillus ochraceus VKM F-4104D. The properties of this protein are similar to those of the protein-C activator from the venom of the snake Agkistrodon contortrix contortrix, which is used in modern laboratory diagnostics of protein C. The method combines fractionation by ammonium sulfate and hydrophobic, ion-exchange, and gel-permeation chromatography. As a result, a highly purified preparation of protein-C activator was obtained with a 350-fold increase in specific activity during the purification process.

中文翻译：

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my曲霉VKM F-4104D产生的人血浆蛋白C蛋白酶活化剂的纯化

摘要

已经开发出一种方法，用于从och曲霉曲霉VKM F-4104D的培养液中纯化人血浆中的蛋白C的蛋白酶活化剂。这种蛋白质的性质类似于从蛇毒液那些蛋白质-C活化剂的铜头蝮contortrix，其在蛋白C的现代实验室诊断由硫酸铵和疏水，离子交换的方法结合使用的分馏，和凝胶渗透色谱。结果，获得了高度纯化的蛋白-C活化剂制剂，在纯化过程中其比活性提高了350倍。