Abstract
A method has been developed for the purification of a protease activator of protein C in human blood plasma from the culture fluid of micromycete Aspergillus ochraceus VKM F-4104D. The properties of this protein are similar to those of the protein-C activator from the venom of the snake Agkistrodon contortrix contortrix, which is used in modern laboratory diagnostics of protein C. The method combines fractionation by ammonium sulfate and hydrophobic, ion-exchange, and gel-permeation chromatography. As a result, a highly purified preparation of protein-C activator was obtained with a 350-fold increase in specific activity during the purification process.
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Komarevtsev, S.K., Popova, E.A., Kreyer, V.G. et al. Purification of the Protease Activator of Protein C of Human Blood Plasma Produced by the Micromycete Aspergillus ochraceus VKM F-4104D. Appl Biochem Microbiol 56, 32–36 (2020). https://doi.org/10.1134/S0003683820010093
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DOI: https://doi.org/10.1134/S0003683820010093