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Bionic composite hydrogel with a hybrid covalent/noncovalent network promoting phenotypic maintenance of hyaline cartilage.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-03-17 , DOI: 10.1039/d0tb00253d Qing Wang 1 , Xing Li 1 , Peilei Wang 1 , Ya Yao 1 , Yang Xu 1 , Yafang Chen 1 , Yong Sun 1 , Qing Jiang 1 , Yujiang Fan 1 , Xingdong Zhang 1
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-03-17 , DOI: 10.1039/d0tb00253d Qing Wang 1 , Xing Li 1 , Peilei Wang 1 , Ya Yao 1 , Yang Xu 1 , Yafang Chen 1 , Yong Sun 1 , Qing Jiang 1 , Yujiang Fan 1 , Xingdong Zhang 1
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The injectable composite hydrogel based on collagen and hyaluronic acid provided a bionic three-dimensional microenvironment and mimetic natural extracellular matrix (ECM) for the growth of cells in vivo and has been widely researched and developed for cartilage tissue engineering. Here, a novel injectable bionic hydrogel with hybrid covalent/noncovalent network derived from covalent conjugation of HA-SH and noncovalent supramolecular self-assembly of BPAA-AFF-OH short peptide was fabricated to overcome the collagen immunogenicity of animal origin and effectively maintain its biological function. Moreover, through optimizing the network structure and polymer composition, the bionic HS5FFAB5 hydrogel presented a reliable mechanical strength which depended on the highly integrated fiber structure between HA-SH and FFAB-AFF-OH molecules. The results in vitro and in vivo proved that HA-SH could provide a fundamental frame structure, while the supramolecular hydrogels could reinforce this structure via hydrogen bonds and hydrophilic/hydrophobic interactions, and endow bionic hydrogels with more abundant cell adhesion sites. The bionic composite hydrogel could improve the cell adhesion and proliferation when compared to HA-SH hydrogel, and enhanced chondrogenic related gene expression and matrix secretion by three-dimensional co-cultured in vitro and subcutaneous implantation in vivo, which further promoted phenotypic maintenance of hyaline cartilage. This bionic hydrogel with a hybrid covalent/noncovalent network is supposed to have potential application prospects in cartilage regeneration.
中文翻译：
具有混合共价/非共价网络的仿生复合水凝胶，可促进透明软骨的表型维持。
基于胶原蛋白和透明质酸的可注射复合水凝胶为体内细胞的生长提供了仿生的三维微环境和模拟的天然细胞外基质（ECM），并已被广泛研究和开发用于软骨组织工程。在这里，一种新颖的可注射的仿生水凝胶具有混合共价/非共价网络，该网络由HA-SH的共价结合和BPAA-AFF-OH短肽的非共价超分子自组装而得，从而克服了动物来源的胶原蛋白的免疫原性并有效地维持了其生物学性功能。此外，通过优化网络结构和聚合物组成，仿生HS 5 FFAB 5水凝胶具有可靠的机械强度，这取决于HA-SH和FFAB-AFF-OH分子之间的高度集成的纤维结构。结果在体外和体内证明，HA-SH可以提供一个基本的框架结构，而超分子水凝胶可以加强该结构通过氢键和亲水/疏水相互作用，以及赋予更丰富的细胞粘附位点仿生水凝胶。相比HA-SH的水凝胶时的仿生复合水凝胶可以提高细胞的粘附和增殖，并通过三维共培养增强软骨相关基因的表达和分泌基质体外和皮下植入体内，这进一步促进了透明软骨的表型维持。这种具有混合共价/非共价网络的仿生水凝胶被认为在软骨再生中具有潜在的应用前景。
更新日期：2020-03-17
中文翻译：
具有混合共价/非共价网络的仿生复合水凝胶，可促进透明软骨的表型维持。
基于胶原蛋白和透明质酸的可注射复合水凝胶为体内细胞的生长提供了仿生的三维微环境和模拟的天然细胞外基质（ECM），并已被广泛研究和开发用于软骨组织工程。在这里，一种新颖的可注射的仿生水凝胶具有混合共价/非共价网络，该网络由HA-SH的共价结合和BPAA-AFF-OH短肽的非共价超分子自组装而得，从而克服了动物来源的胶原蛋白的免疫原性并有效地维持了其生物学性功能。此外，通过优化网络结构和聚合物组成，仿生HS 5 FFAB 5水凝胶具有可靠的机械强度，这取决于HA-SH和FFAB-AFF-OH分子之间的高度集成的纤维结构。结果在体外和体内证明，HA-SH可以提供一个基本的框架结构，而超分子水凝胶可以加强该结构通过氢键和亲水/疏水相互作用，以及赋予更丰富的细胞粘附位点仿生水凝胶。相比HA-SH的水凝胶时的仿生复合水凝胶可以提高细胞的粘附和增殖，并通过三维共培养增强软骨相关基因的表达和分泌基质体外和皮下植入体内，这进一步促进了透明软骨的表型维持。这种具有混合共价/非共价网络的仿生水凝胶被认为在软骨再生中具有潜在的应用前景。
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