Short-chain fructooligosaccharides (ScFOS) are a group of linear fructose oligomers that include 1-kestose, 1-nystose and 1-β-fructofuranosylnystose. ScFOS, which naturally occur at low levels in different plant products, are of high interest as food ingredients because of their prebiotic character, organoleptic characteristics and technological properties. Two different industrial processes are used to achieve large-scale ScFOS production: inulin hydrolysis (enzymatic or chemical hydrolysis) or sucrose biotransformation by transfructosylation (enzymatic synthesis) using specific enzymes like fructosyltransferases and fructofuranosidases. Enzymatic ScFOS synthesis seems to be more advantageous than inulin hydrolysis since it is less expensive, and leads to lower molecular weight FOS. The biotechnological process described to carry out this catalysis includes the production of transfructosylation enzymes, separation, enzyme immobilisation and finally the ScFOS production and purification. Such ScFOS production processes may be conducted under submerged or solid-state fermentation under discontinuous or continuous conditions. Several methodologies with different economic/environmental costs and production yields have been described to carry out these ScFOS production stages, although industrial scale-up needs to be optimised. This review tries to address a revision about enzymatic ScFOS production methods and its scale-up to industrial levels.

短链果糖寡糖（ScFOS）是一组线性果糖低聚物，包括1-钾，1-乳糖和1- β-果糖呋喃糖基nystose。天然存在于不同植物产品中的ScFOS含量低，由于其益生元特性，感官特性和技术特性，作为食品成分备受关注。两种不同的工业过程用于实现大规模ScFOS生产：菊粉水解（酶促或化学水解）或使用果糖基转移酶和果糖呋喃糖苷酶等特定酶通过反式果糖基化（酶促合成）进行蔗糖生物转化。酶促ScFOS合成似乎比菊粉水解更有利，因为它便宜，并且导致较低分子量的FOS。进行这种催化作用的生物技术过程包括产生反式果糖基化酶，分离，酶固定化，最后是ScFOS的生产和纯化。这样的ScFOS生产过程可以在不连续或连续条件下的浸没或固态发酵下进行。尽管需要优化工业规模生产，但已经描述了几种具有不同经济/环境成本和产量的方法来进行这些ScFOS生产阶段。这篇评论试图解决关于酶促ScFOS生产方法及其工业规模扩大的修订。尽管需要优化工业规模。这篇评论试图解决关于酶促ScFOS生产方法及其工业规模扩大的修订。尽管需要优化工业规模。这篇评论试图解决关于酶促ScFOS生产方法及其将其扩大到工业水平的修订。