Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use of alternative and ecological carbon sources for yeast growth. In the present study a high-throughput screening comprising 366 distinct yeast isolates was performed by applying an optimised protocol based on two approaches: (I) yeast cultivation on solid medium using acetic acid as carbon source, (II) neutral lipid estimation by fluorimetry using the lipophilic dye Nile red. Results showed that, with the proposed methodology, the oleaginous potential of yeasts with broad taxonomic diversity and variety of growth characteristics was discriminated. Furthermore, this work clearly demonstrated the association of the oleaginous yeast character to the strain level, contrarily to the species-level linkage, as usually stated.

中文翻译：

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改良的高通量尼罗红荧光测定法，用于使用乙酸作为碳源快速筛选含油酵母。

在过去的几年中，已经研究了含油酵母用于多种能量，油化学，医学和药学目的。然而，只有少数酵母是已知的并且已经被深度开发。寻找具有高产油能力的新分离株以及使用替代和生态碳源促进酵母生长变得势在必行。在本研究中，通过应用基于两种方法的优化方案，对包含366种不同酵母分离株的高通量筛选进行了研究：（I）使用乙酸作为碳源在固体培养基上进行酵母培养，（II）使用荧光法通过中性脂质估算亲脂性染料尼罗红。结果表明，采用所提出的方法，可以区分具有广泛分类学多样性和多种生长特性的酵母的油质潜力。