The diagnosis of dengue infection is still a critical factor determining success in the clinical management and treatment of patients. Here, the development of microfluidic paper-based analytical devices (μPADs) utilizing a sandwich immunoassay on wax patterned paper functionalized with anti-dengue NS1 monoclonal antibodies for point-of-care detection of dengue NS1 (DEN-NS1-PAD) is reported. Various assay conditions, including the length of the channel and diluent, were optimized, and the response detected by the naked eye and digitized images within 20-30 min. The DEN-NS1-PAD was successfully tested in the field for detecting dengue NS1 in buffer, cell culture media, and human serum. The limit of detection (LoD) of the DEN-NS1-PAD obtained with the naked eye, scanner, and a smartphone camera was 200, 46.7, and 74.8 ng mL-1, respectively. The repeatability, reproducibility, and stability of the DEN-NS1-PAD were also evaluated. High true specificity and sensitivity in the serum of pediatric patients were observed. These evaluation results confirm that the DEN-NS1-PAD can potentially be used in point-of-care dengue diagnostics, which can significantly impact on the spreading of mosquito-borne diseases, which are likely to become more prevalent with the effects of global warming. Graphical Abstract.

中文翻译：

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使用基于微流纸的分析设​​备检测小儿血清中的登革热NS1。

登革热感染的诊断仍然是决定患者临床管理和治疗成功与否的关键因素。在此，报道了利用在夹有抗登革热NS1单克隆抗体功能化蜡图案纸上进行夹心免疫分析以进行登革热NS1（DEN-NS1-PAD）即时检测的微流体纸质分析设备（μPAD）的开发。优化了各种测定条件，包括通道长度和稀释剂，并在20-30分钟内用肉眼和数字化图像检测了响应。DEN-NS1-PAD已在现场成功测试，可以检测缓冲液，细胞培养基和人血清中的登革热NS1。用肉眼，扫描仪和智能手机相机获得的DEN-NS1-PAD的检出限（LoD）分别为200、46.7和74.8 ng mL-1。还评估了DEN-NS1-PAD的重复性，再现性和稳定性。观察到儿科患者血清中具有很高的真实特异性和敏感性。这些评估结果证实，DEN-NS1-PAD可以潜在地用于即时诊断登革热，这可能会严重影响蚊媒疾病的传播，而随着全球变暖的影响，蚊子传播的疾病可能会变得更加普遍。图形概要。随着全球变暖的影响，这种情况可能会变得更加普遍。图形概要。随着全球变暖的影响，这种情况可能会变得更加普遍。图形概要。