Burns are one of the most common injuries that are complicated by many challenges including infection, severe inflammatory response, excessive expression of proteases, and scar formation. The aim of this study was to investigate the effect of botulinum toxin type A (BO) and aprotinin (AP) separately or in combination (BO-AP) in healing process. Four burn wounds were created in each rat and randomly filled with silver sulfadiazine (SSD), BO, AP and BO-AP. The rats were euthanized after 7, 14, and 28 days, and their harvested wound samples were evaluated by gross pathology, histopathology, gene expression, biochemical testing, and scanning electron microscopy. Both BO and AP significantly reduced expression of interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) at the 7th post wounding day. Moreover, they inhibited scar formation by reducing the TGF-β1 level and increasing basic fibroblast growth factor (bFGF) at the 28th day. AP by decreasing protease production showed more effective role than BO in wound regeneration. AP increased tissue organization and maturation and improved cosmetic appearance of wounds, at 28 days. The best results gained when combination of BO and AP were used in healing of burn wounds. Treatment by BO-AP significantly subsided inflammation compared to the BO, AP, and SSD treated wounds. Treatment with BO-AP also reduced collagen density and led to minimal scar formation. Combination of botulinum toxin type A and aprotinin considerably increased structural and functional properties of the healing wounds by reducing scar formation and decreasing production of proteases.

中文翻译：

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比较动物模型中A型肉毒杆菌毒素和抑肽酶单一疗法与联合疗法在烧伤创面愈合中的作用。

烧伤是最常见的伤害之一，并伴有许多挑战，包括感染，严重的炎症反应，蛋白酶的过度表达和疤痕形成。这项研究的目的是研究A型肉毒毒素（BO）和抑肽酶（AP）分别或联合使用（BO-AP）在愈合过程中的作用。在每只大鼠中产生四个烧伤伤口，并随机填充磺胺嘧啶银（SSD），BO，AP和BO-AP。在7、14、28天后对大鼠实施安乐死，并通过大体病理学，组织病理学，基因表达，生化测试和扫描电子显微镜对收获的伤口样品进行评估。伤后第7天，BO和AP均显着降低白介素-1β（IL-1β）和转化生长因子-β1（TGF-β1）的表达。此外，他们在第28天通过降低TGF-β1水平和增加碱性成纤维细胞生长因子（bFGF）来抑制瘢痕形成。通过减少蛋白酶产生的AP在伤口再生中显示出比BO更有效的作用。在28天时，AP可增加组织的组织和成熟度，并改善伤口的外观。将BO和AP组合用于烧伤创面时可获得最佳效果。与BO，AP和SSD治疗的伤口相比，BO-AP的治疗可明显减轻炎症。BO-AP的治疗还降低了胶原蛋白的密度，并减少了疤痕形成。A型肉毒杆菌毒素和抑肽酶的组合通过减少疤痕的形成和减少蛋白酶的产生，大大提高了愈合伤口的结构和功能特性。