We investigated the molecular epidemiology of respiratory syncytial virus (RSV) isolated from children during 28 consecutive seasons (1990-2018) and the genetic variability of the duplication region of RSV genotypes ON1 and BA in South Korea. RSV was identified using culture-based methods in Hep-2 cells and was grouped as RSV-A or RSV-B by an immunofluorescence assay. The second hypervariable region of the G gene was sequenced for genotyping. The nucleotide and deduced amino acid sequences of the duplication region of RSV ON1 and BA were analyzed. A total of 670 RSV-A and 233 RSV-B isolates were obtained. For RSV-A, the NA1 genotype predominated during the 2004/2005-2011/2012 seasons. The ON1 genotype was first detected in 2011 and has since replaced all other genotypes. For RSV-B, the GB3 genotype predominated during the 1999/2000-2005/2006 seasons, but the BA genotype also replaced all other genotypes of RSV-B after the first season in which it was isolated (2005/2006). In ON1 and BA genotype RSV strains, novel sequence types of the duplication region of the G gene were identified in 50-95% and 33-80% of the isolates, respectively, in each season. The ON1 and BA9 genotypes are responsible for the current epidemics of RSV infection in South Korea. The sequences in the duplication region of the G gene have evolved continuously and might be sufficient for the identification of specific strains of the RSV-A ON1 and RSV-B BA genotypes.

中文翻译：

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呼吸道合胞病毒连续28季（1990-2018）的分子流行病学和韩国基因型ON1和BA的G基因重复区的遗传变异。

我们调查了在连续28个季节（1990-2018年）从儿童中分离出的呼吸道合胞病毒（RSV）的分子流行病学以及韩国RSV基因型ON1和BA的复制区的遗传变异性。使用基于培养的方法在Hep-2细胞中鉴定出RSV，并通过免疫荧光分析将其归类为RSV-A或RSV-B。对G基因的第二个高变区进行测序以进行基因分型。分析了RSV ON1和BA的重复区的核苷酸和推导的氨基酸序列。总共获得了670个RSV-A和233个RSV-B分离株。对于RSV-A，NA1基因型在2004 / 2005-2011 / 2012季节中占主导地位。ON1基因型于2011年首次发现，此后取代了所有其他基因型。对于RSV-B，GB3基因型在1999 / 2000-2005 / 2006季节占主导地位，但BA基因型在分离后的第一个季节（2005/2006）后也取代了RSV-B的所有其他基因型。在ON1和BA基因型RSV菌株中，每个季节分别在50-95％和33-80％的分离物中鉴定出G基因重复区的新型序列类型。ON1和BA9基因型是造成韩国目前RSV感染流行的原因。G基因重复区中的序列已连续进化，可能足以鉴定RSV-A ON1和RSV-B BA基因型的特定菌株。在每个季节中，分别在50-95％和33-80％的分离物中鉴定出G基因重复区的新型序列类型。ON1和BA9基因型是造成韩国目前RSV感染流行的原因。G基因重复区中的序列已连续进化，可能足以鉴定RSV-A ON1和RSV-B BA基因型的特定菌株。在每个季节中，分别在50-95％和33-80％的分离物中鉴定出G基因重复区的新型序列类型。ON1和BA9基因型是造成韩国目前RSV感染流行的原因。G基因重复区中的序列已连续进化，可能足以鉴定RSV-A ON1和RSV-B BA基因型的特定菌株。