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Molecular epidemiology of respiratory syncytial virus for 28 consecutive seasons (1990-2018) and genetic variability of the duplication region in the G gene of genotypes ON1 and BA in South Korea

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Abstract

We investigated the molecular epidemiology of respiratory syncytial virus (RSV) isolated from children during 28 consecutive seasons (1990-2018) and the genetic variability of the duplication region of RSV genotypes ON1 and BA in South Korea. RSV was identified using culture-based methods in Hep-2 cells and was grouped as RSV-A or RSV-B by an immunofluorescence assay. The second hypervariable region of the G gene was sequenced for genotyping. The nucleotide and deduced amino acid sequences of the duplication region of RSV ON1 and BA were analyzed. A total of 670 RSV-A and 233 RSV-B isolates were obtained. For RSV-A, the NA1 genotype predominated during the 2004/2005-2011/2012 seasons. The ON1 genotype was first detected in 2011 and has since replaced all other genotypes. For RSV-B, the GB3 genotype predominated during the 1999/2000-2005/2006 seasons, but the BA genotype also replaced all other genotypes of RSV-B after the first season in which it was isolated (2005/2006). In ON1 and BA genotype RSV strains, novel sequence types of the duplication region of the G gene were identified in 50-95% and 33-80% of the isolates, respectively, in each season. The ON1 and BA9 genotypes are responsible for the current epidemics of RSV infection in South Korea. The sequences in the duplication region of the G gene have evolved continuously and might be sufficient for the identification of specific strains of the RSV-A ON1 and RSV-B BA genotypes.

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The datasets generated and/or analyzed in the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Sun Joong Kim and Sung Yeon Lee for their technical support. This work was supported by grant number 0420170430 (2017-1169) from the SNUH Research Fund.

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Authors and Affiliations

  1. Department of Pediatrics, Seoul National University College of Medicine, Seoul, 03080, South Korea

    Ki Wook Yun, Eun Hwa Choi & Hoan Jong Lee

  2. Department of Pediatrics, Seoul National University Children’s Hospital, Seoul, 03080, South Korea

    Ki Wook Yun, Eun Hwa Choi & Hoan Jong Lee

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  1. Ki Wook Yun
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Correspondence to Hoan Jong Lee.

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This work was supported by grant number 0420170430 (2017-1169) from the SNUH Research Fund.

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Yun, K.W., Choi, E.H. & Lee, H.J. Molecular epidemiology of respiratory syncytial virus for 28 consecutive seasons (1990-2018) and genetic variability of the duplication region in the G gene of genotypes ON1 and BA in South Korea. Arch Virol 165, 1069–1077 (2020). https://doi.org/10.1007/s00705-020-04580-z

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  • DOI: https://doi.org/10.1007/s00705-020-04580-z

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