Abstract

The selective identification and removal of senescent cells including senescent cancer cells are very important to prolong life and improve the treatment efficacy of cancer therapy. In this study, we integrated the high selectivity of enzyme-instructed self-assembly (EISA) and efficient reactive oxygen species (ROS) generating property of a novel luminogen with aggregation-induced emission (AIE) character to selectively identify and remove senescent HeLa (s-HeLa) cells. The s-HeLa cells expressed high levels of β-galactosidase (β-Gal), which led to the selective accumulation and formation of nanomaterials of Comp. 1 in the cells. Upon white light irradiation, the nanomaterials efficiently produced ROS and therefore killed s-HeLa cells. Our study demonstrated a promising strategy to selectively remove senescent cells and improve the treatment efficacy of cancer therapy.

中文翻译：

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β-半乳糖苷酶反应性AIE荧光基因用于鉴定和去除衰老的癌细胞

摘要

选择性鉴定和去除包括衰老癌细胞在内的衰老细胞对于延长寿命和提高癌症治疗的疗效非常重要。在这项研究中，我们结合了酶促自组装（EISA）的高选择性和新型发光剂具有聚集诱导发射（AIE）特性的高效活性氧（ROS）生成特性，以选择性地识别和去除衰老的HeLa（ s-HeLa）细胞。s-HeLa细胞表达高水平的β-半乳糖苷酶（β-Gal），从而导致Comp纳米材料的选择性积累和形成。1个在细胞中。在白光照射下，纳米材料有效地产生了ROS，因此杀死了s-HeLa细胞。我们的研究证明了一种有前途的策略，可以选择性地去除衰老细胞并提高癌症治疗的疗效。