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β-galactosidase responsive AIE fluorogene for identification and removal of senescent cancer cells

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Abstract

The selective identification and removal of senescent cells including senescent cancer cells are very important to prolong life and improve the treatment efficacy of cancer therapy. In this study, we integrated the high selectivity of enzyme-instructed self-assembly (EISA) and efficient reactive oxygen species (ROS) generating property of a novel luminogen with aggregation-induced emission (AIE) character to selectively identify and remove senescent HeLa (s-HeLa) cells. The s-HeLa cells expressed high levels of β-galactosidase (β-Gal), which led to the selective accumulation and formation of nanomaterials of Comp. 1 in the cells. Upon white light irradiation, the nanomaterials efficiently produced ROS and therefore killed s-HeLa cells. Our study demonstrated a promising strategy to selectively remove senescent cells and improve the treatment efficacy of cancer therapy.

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Acknowledgments

This work was supported by the National Key Research and Development Program of China (2017YFC2103502, 2017YFE0132200), the Fundamental Research Funds for the Central Universities, the National Natural Science Fundation of China (31870949, 31670973), and Tianjin Science Fund for Distinguished Young Scholars (17JCJQJC44900).

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, 300071, China

    Zhengfeng Gao & Ling Wang

  2. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin, 300060, China

    Heqi Gao, Debin Zheng, Tengyan Xu, Yaoxia Chen, Chunhui Liang, Dan Ding & Zhimou Yang

Authors
  1. Zhengfeng Gao
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  2. Heqi Gao
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  3. Debin Zheng
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  4. Tengyan Xu
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  5. Yaoxia Chen
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  6. Chunhui Liang
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  7. Ling Wang
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  8. Dan Ding
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  9. Zhimou Yang
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Corresponding authors

Correspondence to Ling Wang, Dan Ding or Zhimou Yang.

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Conflict of interest The authors declare that they have no conflict of interest.

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Gao, Z., Gao, H., Zheng, D. et al. β-galactosidase responsive AIE fluorogene for identification and removal of senescent cancer cells. Sci. China Chem. 63, 398–403 (2020). https://doi.org/10.1007/s11426-019-9659-2

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  • DOI: https://doi.org/10.1007/s11426-019-9659-2

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