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Whole-genome sequence analysis of paris virus 1: a novel member of the genus Potyvirus infecting Paris polyphylla var. yunnanensis.
Archives of Virology ( IF 2.5 ) Pub Date : 2020-02-25 , DOI: 10.1007/s00705-020-04560-3
Lu Chen 1, 2 , Rex Frimpong Anane 3, 4 , Zhe Wang 1, 2 , Linyi Yang 1, 2 , Zeli Chen 2, 5 , Guosong Wen 2, 5 , Mingfu Zhao 1, 2
Affiliation  

The complete genome sequence of a novel potyvirus, tentatively named "paris virus 1" (ParV1, GenBank accession no. MN549985), infecting Paris polyphylla var. yunnanensis was determined in this study. A single large open reading frame (nt 96-9818) encoding a 3240-aa polyprotein that is predicted to be cleaved into 10 mature proteins was detected in the ParV1 genome. Comparative analysis of the ParV1 genome sequence with those of other potyviruses identified nine cleavage sites and conserved motifs that are typical features of potyviruses. Pairwise sequence comparisons showed that the ParV1 polyprotein shares 49.6-65.1% nucleotide and 47.1-68.9% amino acid sequence identity with viruses of the genus Potyvirus. BLAST analysis revealed that ParV1 shares 65.1% nucleotide and 68.9% amino acid sequence identity with Thunberg fritillary mosaic virus (TFMV, accession no. CAI59123), its closest known relative. These results suggest that paris virus 1 (ParV1) is a new member of the genus Potyvirus.

中文翻译:

巴黎病毒1的全基因组序列分析:波多病毒属的一种新型成员,感染巴黎多叶植物var。云南

一种新的马铃薯瘟病毒的完整基因组序列,暂定名为“巴黎病毒1”(ParV1,GenBank登录号为MN549985),感染了巴黎多叶变种。本研究确定了云南。在ParV1基因组中检测到一个编码3240-aa多聚蛋白的单个大开放阅读框(nt 96-9818),该多聚蛋白预计会被切割成10种成熟蛋白。对ParV1基因组序列与其他马铃薯病毒的序列进行比较分析,确定了9个切割位点和保守基序,这是马铃薯病毒的典型特征。配对序列比较显示,ParV1多蛋白与波多病毒属病毒共享49.6-65.1%的核苷酸和47.1-68.9%的氨基酸序列同一性。BLAST分析显示ParV1共有65.1%的核苷酸和68个核苷酸。与Thunberg贝母花叶病毒(TFMV,保藏号CAI59123)具有9%的氨基酸序列同一性,它是其最接近的亲戚。这些结果表明,巴黎病毒1(ParV1)是马铃薯病毒属的新成员。
更新日期:2020-04-06
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