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Whole-genome sequence analysis of paris virus 1: a novel member of the genus Potyvirus infecting Paris polyphylla var. yunnanensis

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Abstract

The complete genome sequence of a novel potyvirus, tentatively named “paris virus 1” (ParV1, GenBank accession no. MN549985), infecting Paris polyphylla var. yunnanensis was determined in this study. A single large open reading frame (nt 96-9818) encoding a 3240-aa polyprotein that is predicted to be cleaved into 10 mature proteins was detected in the ParV1 genome. Comparative analysis of the ParV1 genome sequence with those of other potyviruses identified nine cleavage sites and conserved motifs that are typical features of potyviruses. Pairwise sequence comparisons showed that the ParV1 polyprotein shares 49.6-65.1% nucleotide and 47.1-68.9% amino acid sequence identity with viruses of the genus Potyvirus. BLAST analysis revealed that ParV1 shares 65.1% nucleotide and 68.9% amino acid sequence identity with Thunberg fritillary mosaic virus (TFMV, accession no. CAI59123), its closest known relative. These results suggest that paris virus 1 (ParV1) is a new member of the genus Potyvirus.

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Author information

Author notes

  1. Lu Chen and Rex Frimpong Anane equally contributed to this study.

Authors and Affiliations

  1. Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Lu Chen, Zhe Wang, Linyi Yang & Mingfu Zhao

  2. Research and Development Center for Heath Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, 32 Jiaochang Donglu, Kunming, 650201, Yunnan, China

    Lu Chen, Zhe Wang, Linyi Yang, Zeli Chen, Guosong Wen & Mingfu Zhao

  3. CAS Center for Excellence in Animal Evolution and Genetics, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

  4. State Key Laboratory for Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

  5. College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zeli Chen & Guosong Wen

Authors
  1. Lu Chen
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  2. Rex Frimpong Anane
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  3. Zhe Wang
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  4. Linyi Yang
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  5. Zeli Chen
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  6. Guosong Wen
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  7. Mingfu Zhao
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Corresponding author

Correspondence to Mingfu Zhao.

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Funding

This study was funded by the National Natural Science Foundation of China (81860774).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Data availability

The dataset generated during the current study is available in the GenBank database (accession nos. MN549985 and MN719460).

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Chen, L., Anane, R.F., Wang, Z. et al. Whole-genome sequence analysis of paris virus 1: a novel member of the genus Potyvirus infecting Paris polyphylla var. yunnanensis. Arch Virol 165, 985–988 (2020). https://doi.org/10.1007/s00705-020-04560-3

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  • DOI: https://doi.org/10.1007/s00705-020-04560-3

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