OBJECTIVE To reveal the systematic response of neutrophils to sepsis and to study the hub lncRNAs in sepsis. MATERIALS AND METHODS Neutrophils taken from the femur and tibia of male C57 BL/6 mice were used in this study. And neutrophils were treated for 0 h, 0.5 h, 1 h, and 4 h with or without 1 µg/mL lipopolysaccharide (LPS) for further chip detection. In addition, cecal ligation and perforation were used to simulate sepsis. Here, we used different bioinformatics analyses, including differential expression analysis, weighted gene co-expression network analysis (WGCNA), and gene regulatory network analysis, to analyze the systemic response of neutrophils to sepsis. RESULTS We identified nine modules and found hub lncRNAs in each module. The blue and pink modules were closely related to the inflammatory state of sepsis. Some hub lncRNAs (NONMMUT005259, KnowTID_00004196, and NR_003507) may have functions related to the inflammatory state in sepsis. CONCLUSIONS Based on a new biological approach, our research results revealed the systemic-level response of neutrophils to sepsis and identified several hub lncRNAs with potential regulatory effects on this condition.

中文翻译：

---

败血症过程中嗜中性粒细胞中枢mRNA和长非编码RNA的鉴定和评估。

目的揭示嗜中性粒细胞对脓毒症的系统反应，并研究脓毒症中枢lncRNA。材料与方法本研究使用取自雄性C57 BL / 6小鼠股骨和胫骨的嗜中性粒细胞。然后在有或没有1 µg / mL脂多糖（LPS）的情况下，将中性粒细胞分别处理0 h，0.5 h，1 h和4 h，以进行进一步的芯片检测。另外，盲肠结扎和穿孔用于模拟败血症。在这里，我们使用了不同的生物信息学分析，包括差异表达分析，加权基因共表达网络分析（WGCNA）和基因调控网络分析，以分析嗜中性粒细胞对败血症的全身反应。结果我们确定了9个模块，并在每个模块中找到了枢纽lncRNA。蓝色和粉红色的模块与脓毒症的炎症状态密切相关。一些集线器lncRNA（NONMMUT005259，KnowTID_00004196和NR_003507）可能具有与脓毒症中炎症状态相关的功能。结论基于一种新的生物学方法，我们的研究结果揭示了嗜中性粒细胞对败血症的全身水平反应，并鉴定了几种在这种情况下具有潜在调节作用的中枢lncRNA。