Lactobionic acid (LBA) was produced by fermentation of Pseudomonas taetrolens. First, to increase the production of LBA by P. taetrolens, we controlled the pH of culture medium by CaCO3 addition (30 g/L) and then examined the initial lactose concentration ranging from 50 to 200 g/L and the growth temperature ranging from 20 to 37 °C. Both the LBA production titer (180 g/L) and the productivity (2.5 g/L h) were highest at 200 g/L lactose concentration and 25 °C of cell growth temperature in shake-flask culture. Although the production of LBA (178 g/L) was almost similar during the batch fermentation of P. taetrolens using 5 L bioreactor, the LBA productivity highly increased to 4.9 g/L h. The method using ethanol precipitation and ion-exchange chromatography was developed to recover the pure LBA from the fermentation broth. The optimum volume of ethanol and pH of culture medium for the precipitation of Ca2+ salt form of LBA were six volume of ethanol and pH 6.5, respectively. The cation-exchange resin T42 finally showed the best recovery yield (97.6%) of LBA from the culture supernatant. The production titer (178 g/L) and the productivity (4.9 g/L h) of lactobionic acid in this study were highest among the previous studies ever reported using P. taetrolens as a production strain of LBA.

中文翻译：

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通过控制假单胞菌的发酵过程中的pH和温度来高产和高收率回收乳糖酸。

乳酸假单胞菌酸（LBA）是通过发酵假单胞菌产生的。首先，为了增加ta。taetrolens产生的LBA，我们通过添加CaCO3（30 g / L）来控制培养基的pH，然后检查初始乳糖浓度为50至200 g / L，并且生长温度为20至37°C。在摇瓶培养中，乳糖浓度为200 g / L且细胞生长温度为25°C时，LBA的生产效价（180 g / L）和生产率（2.5 g / L h）最高。尽管在使用5 L生物反应器分批发酵taetrolens的过程中LBA的产量（178 g / L）几乎相似，但LBA的生产率却大大提高到4.9 g / L h。建立了使用乙醇沉淀和离子交换色谱法从发酵液中回收纯LBA的方法。用于沉淀LBA的Ca2 +盐形式的最适乙醇体积和培养基pH分别为6体积乙醇和pH 6.5。阳离子交换树脂T42最终显示出从培养上清液中的LBA的最佳回收率（97.6％）。该研究中乳糖酸的生产效价（178 g / L）和生产能力（4.9 g / L h）在使用taetrolens作为LBA生产菌株的以往研究中最高。