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High-level production and high-yield recovery of lactobionic acid by the control of pH and temperature in fermentation of Pseudomonas taetrolens

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Abstract

Lactobionic acid (LBA) was produced by fermentation of Pseudomonas taetrolens. First, to increase the production of LBA by P. taetrolens, we controlled the pH of culture medium by CaCO3 addition (30 g/L) and then examined the initial lactose concentration ranging from 50 to 200 g/L and the growth temperature ranging from 20 to 37 °C. Both the LBA production titer (180 g/L) and the productivity (2.5 g/L h) were highest at 200 g/L lactose concentration and 25 °C of cell growth temperature in shake-flask culture. Although the production of LBA (178 g/L) was almost similar during the batch fermentation of P. taetrolens using 5 L bioreactor, the LBA productivity highly increased to 4.9 g/L h. The method using ethanol precipitation and ion-exchange chromatography was developed to recover the pure LBA from the fermentation broth. The optimum volume of ethanol and pH of culture medium for the precipitation of Ca2+ salt form of LBA were six volume of ethanol and pH 6.5, respectively. The cation-exchange resin T42 finally showed the best recovery yield (97.6%) of LBA from the culture supernatant. The production titer (178 g/L) and the productivity (4.9 g/L h) of lactobionic acid in this study were highest among the previous studies ever reported using P. taetrolens as a production strain of LBA.

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Acknowledgements

This work was supported in part by the R&D program of MOTIE/KEIT (10077291), in part by the R&D program of MOTIE/KEIT (10080592), and in part by the R&D program of KRICT (SI1809).

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Authors and Affiliations

  1. Bio-based Chemistry Research Center, Korea Research Institute of Chemical Technology (KRICT), 406-30 Jongga-ro, Ulsan, 44429, Republic of Korea

    Jung-Hun Kim, Young-Ah Jang, Si-Bum Seong & Gyeong Tae Eom

  2. Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 305-350, Republic of Korea

    Si-Bum Seong & Gyeong Tae Eom

  3. Bio-based Chemistry Research Center, Korea Research Institute of Chemical Technology (KRICT), 141 Gajeong-ro, Daejeon, 34114, Republic of Korea

    Sun Ah Jang & Jae Kwang Song

  4. School of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan, 680-749, Republic of Korea

    Soon Ho Hong

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  1. Jung-Hun Kim
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  2. Young-Ah Jang
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  3. Si-Bum Seong
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  4. Sun Ah Jang
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Correspondence to Jae Kwang Song or Gyeong Tae Eom.

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Kim, JH., Jang, YA., Seong, SB. et al. High-level production and high-yield recovery of lactobionic acid by the control of pH and temperature in fermentation of Pseudomonas taetrolens. Bioprocess Biosyst Eng 43, 937–944 (2020). https://doi.org/10.1007/s00449-020-02290-z

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  • DOI: https://doi.org/10.1007/s00449-020-02290-z

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