This study describes two Bradyrhizobium strains, UFLA03-164T and UFLA03-153, which share more than 99% sequence similarity of the 16S rRNA with the type strains of 15 species in this genus. The concatenation of three housekeeping genes (recA, gyrB, and dnaK) indicated that both strains formed a single clade separate from known Bradyrhizobium species. B. viridifuturi, represented by SEMIA 690T, is the closest neighboring species (96.2%). Low (< 92%) average nucleotide identity (ANI) was observed between strain UFLA03-164T and any of the closest species on the phylogenetic trees based on concatenated housekeeping genes. The DNA G+C content of UFLA03-164T is 63.25%. Phenotypic characteristics were determined for both UFLA strains. Based on the data, the two strains represent a new species for which the name Bradyrhizobium uaiense is proposed, with UFLA03-164T (= LMG 31509T) as type strain.

中文翻译：

---

Bradyrhizobium uaiense sp. 11月，一种新型高效豇豆共生体

本研究描述了两种慢生根瘤菌菌株，UFLA03-164T 和 UFLA03-153，它们与该属 15 个物种的类型菌株的 16S rRNA 具有超过 99% 的序列相似性。三个管家基因（recA、gyrB 和 dnaK）的串联表明这两个菌株形成了一个独立于已知慢生根瘤菌物种的进化枝。B. viridifuturi，以SEMIA 690T为代表，是最近的邻近物种（96.2%）。在菌株 UFLA03-164T 与基于串联看家基因的系统发育树上任何最接近的物种之间观察到低 (< 92%) 平均核苷酸同一性 (ANI)。UFLA03-164T的DNA G+C含量为63.25%。确定了两种 UFLA 菌株的表型特征。根据数据，这两个菌株代表了一个新物种，提议命名为 Bradyrhizobium uaiense，