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Bradyrhizobium uaiense sp. nov., a new highly efficient cowpea symbiont

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Abstract

This study describes two Bradyrhizobium strains, UFLA03-164T and UFLA03-153, which share more than 99% sequence similarity of the 16S rRNA with the type strains of 15 species in this genus. The concatenation of three housekeeping genes (recA, gyrB, and dnaK) indicated that both strains formed a single clade separate from known Bradyrhizobium species. B. viridifuturi, represented by SEMIA 690T, is the closest neighboring species (96.2%). Low (< 92%) average nucleotide identity (ANI) was observed between strain UFLA03-164T and any of the closest species on the phylogenetic trees based on concatenated housekeeping genes. The DNA G+C content of UFLA03-164T is 63.25%. Phenotypic characteristics were determined for both UFLA strains. Based on the data, the two strains represent a new species for which the name Bradyrhizobium uaiense is proposed, with UFLA03-164T (= LMG 31509T) as type strain.

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Acknowledgements

We thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (CAPES/PROEX AUXPE 593/2018), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Process: 304527/2016-5; Process: 431504/2016-4), and the Fundação de Amparo e Pesquisa de Minas Gerais (Fapemig) (PACCSS/PPGCS-2009-2012) for financial support and for granting scholarships. This research is associated with the Brazilian National Institute of Science and Technology (Soil Biodiversity/INCT-CNPq).

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Authors and Affiliations

  1. Setor de Biologia, Microbiologia e Processos Biológicos Do Solo, Departamento de Ciência Do Solo, Universidade Federal de Lavras, Campus UFLA, Lavras, Minas Gerais, 37200-000, Brazil

    Daniele Cabral Michel, Amanda Azarias Guimarães, Teotonio Soares de Carvalho & Fatima Maria de Souza Moreira

  2. Universidade Federal Do Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, Piauí, 64900-000, Brazil

    Elaine Martins da Costa

  3. Departamento de Bioquímica E Biologia Molecular, Universidade Federal Do Paraná, Curitiba, Paraná, 81531990, Brazil

    Eduardo Balsanelli & Emanuel Maltempi de Souza

  4. Department of Biochemistry and Microbiology, Laboratory of Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium

    Anne Willems

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  1. Daniele Cabral Michel
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  2. Amanda Azarias Guimarães
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Correspondence to Fatima Maria de Souza Moreira.

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Communicated by Erko Stackebrandt.

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Cabral Michel, D., Azarias Guimarães, A., Martins da Costa, E. et al. Bradyrhizobium uaiense sp. nov., a new highly efficient cowpea symbiont. Arch Microbiol 202, 1135–1141 (2020). https://doi.org/10.1007/s00203-020-01827-w

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