Serine/arginine (SR) proteins play significant roles in pre-mRNA splicing in eukaryotes. To investigate how gene expression influences fungal development and pathogenicity in Fusarium graminearum, a causal agent of Fusarium head blight (FHB) of wheat and barley, our previous study identified a SR protein FgSrp1 in F. graminearum, and showed that it is important for conidiation, plant infection and pre-mRNA processing. In this study, we identified another SR protein FgSrp2 in F. graminearum, which is orthologous to Schizosaccharomyces pombe Srp2. Our data showed that, whereas yeast Srp2 is essential for growth, deletion of FgSRP2 resulted in only slight defects in vegetative growth and perithecia melanization. FgSrp2 localized to the nucleus and both its N- and C-terminal regions were important for the localization to the nucleus. FgSrp2 interacted with FgSrp1 to form a complex in vivo. Double deletion of FgSRP1 and FgSRP2 revealed that they had overlapping functions in vegetative growth and sexual reproduction. RNA-seq analysis revealed that, although deletion of FgSRP2 alone had minimal effects, deletion of both FgSRP1 and FgSRP2 caused significant changes in gene transcription and RNA splicing. Overall, our results indicated that FgSrp2 regulates vegetative growth, sexual reproduction and pre-mRNA processing by interacting with FgSrp1.

中文翻译：

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SR蛋白FgSrp2通过与禾谷镰刀菌（Fusarium graminearum）中的FgSrp1相互作用来调节营养生长，有性繁殖和mRNA前加工。

丝氨酸/精氨酸（SR）蛋白在真核生物的mRNA前剪接中起重要作用。为了研究基因表达如何影响小麦和大麦镰刀菌枯萎病（FHB）的致病因子镰刀镰刀菌的真菌发育和致病性，我们先前的研究在镰刀镰刀菌中鉴定了SR蛋白FgSrp1，并表明它对于分生孢子很重要。 ，植物感染和mRNA预处理。在这项研究中，我们在禾谷镰刀菌中鉴定了另一种SR蛋白FgSrp2，它与粟酒裂殖酵母Srp2同源。我们的数据表明，尽管酵母Srp2是生长所必需的，但FgSRP2的缺失仅导致营养生长和皮膜黑化的轻微缺陷。FgSrp2定位于原子核，其N端和C端区域对于定位于原子核都很重要。FgSrp2与FgSrp1相互作用，在体内形成复合物。FgSRP1和FgSRP2的双重删除表明它们在营养生长和有性生殖中具有重叠的功能。RNA-seq分析显示，尽管仅删除FgSRP2的影响很小，但删除FgSRP1和FgSRP2都会引起基因转录和RNA剪接的显着变化。总体而言，我们的结果表明FgSrp2通过与FgSrp1相互作用来调节营养生长，有性繁殖和mRNA前加工。FgSRP1和FgSRP2的缺失引起基因转录和RNA剪接的显着变化。总体而言，我们的结果表明FgSrp2通过与FgSrp1相互作用来调节营养生长，有性生殖和前mRNA加工。FgSRP1和FgSRP2的缺失引起基因转录和RNA剪接的显着变化。总体而言，我们的结果表明FgSrp2通过与FgSrp1相互作用来调节营养生长，有性繁殖和mRNA前加工。