In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. We show that single chain variable fragment (scFv) libraries with adequate qualities can readily be cloned in a 'scar-less' manner and that the isolation of antigen-specific antibodies from immunized chickens is feasible within three selection rounds. Moreover, we demonstrate the general applicability of this method by rapidly constructing and panning VHH single domain antibody phage display libraries from immunized Llama repertoires.

中文翻译：

---

构建噬菌体展示scFv和VHH库的一步式过程。

在这项工作中，我们提出了一种依靠II型限制酶建立抗体噬菌体展示文库的一步克隆方法。我们显示具有足够质量的单链可变片段（scFv）库可以很容易地以“无疤痕”的方式克隆，并且从免疫鸡中分离抗原特异性抗体在三轮选择中是可行的。此外，我们通过从免疫的骆驼库快速构建和淘选VHH单域抗体噬菌体展示文库，证明了该方法的一般适用性。