To determine bacteriocin producers and the prevalence of structural enterocin genes and to detect the spectrum of activity against foodborne pathogens, from isolates of Enterococcus faecium and Enterococcus faecalis that were isolated from food and the environment. The entA, entB, entP, ent1071 and entX genes, which encode enterocins were the most frequently observed. Enterocins were thermostable, proteinaceous, and resistant to catalase. None of the isolates produced hemolysin, and inhibition resulting from bacteriophage lysis was excluded. The bactericidal effect of enterocins against L. innocua 12612 was determined by optical density and colony forming units. For the activity spectrum, elimination of mainly Listeria strains, Bacillus sp. and clinical enterococci, was observed. Imaging with scanning electron microscopy after treatment with enterocin Efm22 showed irregular rod-shaped cells and loss of cellular integrity. The isolates evaluated in this study are candidates for the production of enterocins that will be used as food biopreservatives, because they have high anti-listerial activity even after 24 h of experimentation, and used in the pharmaceutical area because they inhibit clinical microorganisms.

中文翻译：

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肠毒素对李斯特菌的抗菌活性。和其他食物腐败细菌

确定细菌素生产者和结构性肠毒素基因的流行率，并从从食物和环境中分离的屎肠球菌和粪肠球菌的分离物中检测针对食源性病原体的活性谱。编码肠毒素的 entA、entB、entP、ent1071 和 entX 基因是最常观察到的。肠菌素是热稳定的、蛋白质性质的并且对过氧化氢酶具有抗性。没有分离株产生溶血素，排除了噬菌体裂解引起的抑制作用。肠素对无害李斯特菌 12612 的杀菌作用由光密度和菌落形成单位确定。对于活性谱，主要消除李斯特菌菌株、芽孢杆菌属。并观察到临床肠球菌。用肠菌素 Efm22 处理后的扫描电子显微镜成像显示不规则的杆状细胞和细胞完整性的丧失。本研究中评估的分离株是生产肠毒素的候选物，可用作食品生物防腐剂，因为即使经过 24 小时的实验，它们仍具有很高的抗李斯特菌活性，并用于制药领域，因为它们可抑制临床微生物。