Yeast Candida utilis is considered to be a potentially advantageous expression system for production of recombinant proteins utilizable for industrial and pharmaceutical purposes. As the scientific literature is not consistent in the ploidy of this yeast, in this work, we focused on resolving the problem via several methods such as the copy number determination of maltase gene by multiplex PCR, measuring α-glucosidase activity, the characterization of maltase gene copy number in deletion mutants using qPCR and flow cytometry. In context with the published data and results obtained in this study about the copy number of the maltase gene on C. utilis genome, we attempted to hypothesise and made conclusion about the ploidy of C. utilis. The results of this work, besides the biotechnological aspect, contribute to the elementary knowledge of C. utilis. The exact information about the ploidy or more specifically about the copy number of appropriate gene is essential for expression cassette dosage determination integrated into the chromosome of the host. In this study, we come to the conclusion that the maltase gene is present in C. utilis genome in four alleles, and in combination with flow cytometry, published information and the published genome sequences, the observations support the theory about tetraploidy of C. utilis.

中文翻译：

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生物技术上重要的酵母假丝酵母的倍性测定。

酵母菌假丝酵母被认为是用于生产可用于工业和制药目的重组蛋白的潜在有利的表达系统。由于科学文献中关于该酵母的倍性不一致，在这项工作中，我们集中于通过多种方法解决该问题，例如通过多重PCR确定麦芽糖酶基因的拷贝数，测量α-葡萄糖苷酶活性，麦芽糖酶的表征使用qPCR和流式细胞仪检测缺失突变体中的基因拷贝数。与已发表的数据和本研究中有关麦芽糖酶基因在C上的拷贝数的结果有关。utilis基因组，我们尝试进行假设并得出有关C倍性的结论。utilis。这项工作的结果，除了生物技术方面，还有助于C的基本知识。utilis。有关倍性的确切信息，或更确切地说，有关适当基因的拷贝数的确切信息，对于整合入宿主染色体的表达盒剂量确定至关重要。在这项研究中，我们得出的结论是麦芽糖酶基因存在于C中。四个等位基因中的鱼基因组，并结合流式细胞仪，公开的信息和公开的基因组序列，这些观察结果支持了C四倍体的理论。utilis。