Recently, the microwell has been widely used for cell trapping due to its simple design and enclosed microenvironment for on-chip cell culture and stimulation. In this paper, we investigated the effect of various geometrical factors on microwells for efficient particle analysis. We used the Arbitrary Lagrangian–Eulerian method to calculate the trajectory of particles entering circular and triangular microwells under various geometrical factors, particle size, and flow conditions. Our simulation results show that the W/L = 2 triangular microwell provides the best trapping efficiency due to a stronger recirculation vortex. A smaller particle size or slower flow rate also enhances particle trapping efficiency. To validate simulation results, we flowed 4.5, 6, and 10 µm diameter polystyrene beads into W/L = 1 circular, W/L = 1 and W/L = 2 triangular microwells under various flow rates. The experimental results agreed well with simulation results, showing that the occupancy of W/L = 2 triangular microwell was sevenfold and twofold higher than W/L = 1 circular and W/L = 1 triangular microwells, respectively. Overall, the above results can provide a useful guideline to design the microwell device for efficient hydrodynamic particle trapping, which can be applied to single cell analysis or rare cell capture.

最近，由于微孔的简单设计和用于芯片上细胞培养和刺激的封闭微环境，微孔已广泛用于细胞捕获。在本文中，我们研究了各种几何因素对微孔的影响，以进行有效的颗粒分析。我们使用任意的拉格朗日-欧拉方法来计算在各种几何因素，粒径和流动条件下进入圆形和三角形微孔的粒子的轨迹。我们的仿真结果表明，W / L = 2个三角形微孔由于较强的再循环涡流而提供了最佳的捕获效率。较小的粒径或较慢的流速也会提高颗粒捕集效率。为了验证仿真结果，我们 在各种流速下将4.5、6和10 µm直径的聚苯乙烯珠粒流入W / L  = 1圆形，W / L  = 1和W / L = 2三角形微孔中。实验结果与仿真结果吻合良好，表明W / L  = 2三角形微孔的占有率分别比W / L  = 1圆形和W / L高7倍和两倍。L  = 1个三角形微孔。总体而言，以上结果可为设计用于高效流体动力学颗粒捕获的微孔设备提供有用的指导，可将其应用于单细胞分析或稀有细胞捕获。