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High-throughput dynamical analysis of dielectrophoretic frequency dispersion of single cells based on deflected flow streamlines.
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2020-03-04 , DOI: 10.1007/s00216-020-02467-1
Karina Torres-Castro 1 , Carlos Honrado 1 , Walter B Varhue 1 , Vahid Farmehini 1 , Nathan S Swami 1, 2
Affiliation  

Phenotypic quantification of cells based on their plasma membrane capacitance and cytoplasmic conductivity, as determined by their dielectrophoretic frequency dispersion, is often used as a marker for their biological function. However, due to the prevalence of phenotypic heterogeneity in many biological systems of interest, there is a need for methods capable of determining the dielectrophoretic dispersion of single cells at high throughput and without the need for sample dilution. We present a microfluidic device methodology wherein localized constrictions in the microchannel are used to enhance the field delivered by adjoining planar electrodes, so that the dielectrophoresis level and direction on flow-focused cells can be determined on each traversing cell in a high-throughput manner based on their deflected flow streamlines. Using a sample of human red blood cells diluted to 2.25 × 108 cells/mL, the dielectrophoretic translation of single cells traversing at a flow rate of 1.68 μL/min is measured at a throughput of 1.1 × 105 cells/min, to distinguish positive versus negative dielectrophoresis and determine their crossover frequency in media of differing conductivity for validation of the computed membrane capacitance to that from prior methods. We envision application of this dynamic dielectrophoresis (Dy-DEP) method towards high-throughput measurement of the dielectric dispersion of single cells to stratify phenotypic heterogeneity of a particular sample based on their DEP crossover frequency, without the need for significant sample dilution. Grapical abstract.

中文翻译:

基于偏转流线的单细胞介电泳频率色散的高通量动力学分析。

基于细胞的质膜电容和细胞质电导率的细胞的表型定量,通常由其介电电泳频率分散确定,通常用作其生物学功能的标记。然而,由于在许多感兴趣的生物系统中普遍存在表型异质性,因此需要能够以高通量确定单细胞的介电泳分散而不需要稀释样品的方法。我们提出了一种微流控设备方法,其中使用微通道中的局部收缩来增强相邻平面电极所传递的电场,从而可以以高通量的方式基于每个通行细胞确定流集中细胞的介电泳水平和方向在其偏转的流线上。使用稀释至2.25×108细胞/ mL的人类红细胞样品,以1.1×105细胞/ min的通量测量以1.68μL/ min的流速通过的单细胞的介电泳翻译,以区分阳性还是阴性负介电电泳,并在不同电导率的介质中确定其交叉频率,以验证计算出的膜电容与先前方法的电导率。我们设想将这种动态介电电泳(Dy-DEP)方法应用于高通量测量单个细胞的介电弥散度,以便根据其DEP穿越频率对特定样品的表型异质性进行分层,而无需大量稀释样品。语法摘要。以1.1×105个细胞/分钟的通量测量以1.68μL/ min的流速穿过的单个细胞的介电泳翻译,以区分正介电电泳和负介电电泳,并确定其在不同电导率的介质中的交叉频率,以验证计算结果膜电容与以前的方法相比 我们设想将这种动态介电电泳(Dy-DEP)方法应用于高通量测量单个细胞的介电弥散度,以便根据其DEP穿越频率对特定样品的表型异质性进行分层,而无需大量稀释样品。语法摘要。以1.1×105个细胞/分钟的通量测量以1.68μL/ min的流速穿过的单个细胞的介电泳翻译,以区分正介电电泳和负介电电泳,并确定其在不同电导率的介质中的交叉频率,以验证计算结果膜电容与以前的方法相同。我们设想将这种动态介电电泳(Dy-DEP)方法应用于高通量测量单个细胞的介电弥散度,以便根据其DEP穿越频率对特定样品的表型异质性进行分层,而无需大量稀释样品。语法摘要。区分正介电电泳和负介电电泳,并确定在不同电导率的介质中的交叉频率,以验证计算出的膜电容与先前方法相比。我们设想将这种动态介电电泳(Dy-DEP)方法应用于高通量测量单个细胞的介电弥散度,以便根据其DEP穿越频率对特定样品的表型异质性进行分层,而无需大量稀释样品。语法摘要。区分正介电电泳和负介电电泳,并确定在不同电导率的介质中的交叉频率,以验证计算出的膜电容与先前方法相比。我们设想将这种动态介电电泳(Dy-DEP)方法应用于高通量测量单个细胞的介电弥散度,以便根据其DEP穿越频率对特定样品的表型异质性进行分层,而无需大量稀释样品。语法摘要。我们设想将这种动态介电电泳(Dy-DEP)方法应用于高通量测量单个细胞的介电弥散度,以便根据其DEP穿越频率对特定样品的表型异质性进行分层,而无需大量稀释样品。语法摘要。我们设想将这种动态介电电泳(Dy-DEP)方法应用于高通量测量单个细胞的介电弥散度,以便根据其DEP穿越频率对特定样品的表型异质性进行分层,而无需大量稀释样品。语法摘要。
更新日期:2020-03-04
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