Silicosis is one of the prolonged and irreversible occupational diseases. Crystalline silica dust, which has been linked with silicosis, occurs in different industrial areas such as constructions, ceramic, quarry, and pottery. There are significant numbers of newly diagnosed cases every year in Turkey. Patients with silicosis suffer from inflammatory respiratory disorders and silicosis-related complications such as rheumatoid arthritis, systemic sclerosis, and vasculitis. Oxysterols are defined as 27-carbon intermediates or end products of cholesterol. They are also implicated in the etiology of disease states such as atherosclerosis, neurodegenerative, and inflammatory diseases. The aim of the study is to evaluate cholesterol oxidation products in the patients with silicosis and determination of sphingosine-1-phosphate (S1P) levels which is a sphingolipid metabolite. In addition to these parameters, it is aimed to determine the possible lipid peroxidation by different parameters. For this purpose, blood samples and urine were collected from 47 patients and 30 healthy individual with their consents. In order to evaluate oxysterols, 7-ketocholesterol and cholestan 3β,5α,6β-triol levels were measured by LC-MS/MS method. The measured levels of 7-KC were 0.101 ± 0.005 µmol/l in patient and 0.050 ± 0.003 µmol/l in control plasma samples. Triol levels were measured as 0.038 ± 0.005 µmol/l in patient group and 0.033 ± 0.004 µmol/l in control group (p < 0.001). In addition, lipid peroxidation products were measured by human-8-isoprostane, human-4-hydroxynonenal (4-HNE), and human malondialdehyde (MDA) ELISA kits. The measured levels of HNE in the patient and control groups were 735.14 ± 288.80 pg/ml and 595.72 ± 108.62 pg/ml in plasma and 606.02 + 118.23 pg/ml and 531.84 + 107.18 pg/ml in urine, respectively (p < 0.05). F2-iP results of patients and controls were 450.0 + 101.40 pg/dl and 386.9 + 112.7 pg/ml for urine and 432.7 ± 188,8 pg/dl and 321.9 ± 69.4 pg/dl for plasma, respectively (p < 0.05). MDA levels of plasma were measured as 44.1 ± 14.6 nmol/ml in the patient and 31.9 ± 10.5 nmol/ml in the control (p < 0.05). Levels of MDA for urine samples were 30.15 + 5.06 nmol/ml and 25.15 + 6.07 nmol/ml in patients and controls, respectively (p < 0.05). S1P levels were decreased in patients compared to control group (49.05 ± 10.87 and 67.57 ± 16.25, p < 0.001). The results not only indicate a correlation between cholesterol oxidation, lipid peroxidation, and silicosis, but also provide better understanding of the role of the lipids in the mechanism of this inflammatory disease.

中文翻译：

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通过LC-MS / MS方法评估矽肺患者的氧固醇水平。

矽肺病是长期且不可逆的职业病之一。与矽肺病有关的结晶二氧化硅粉尘出现在不同的工业领域，例如建筑，陶瓷，采石场和陶器。土耳其每年都有大量新诊断的病例。矽肺病患者患有炎症性呼吸系统疾病和矽肺病相关的并发症，例如类风湿关节炎，全身性硬化症和血管炎。氧固醇定义为27碳的胆固醇中间体或终产物。它们还与诸如动脉粥样硬化，神经退行性疾病和炎性疾病等疾病状态的病因有关。这项研究的目的是评估矽肺患者的胆固醇氧化产物，并确定鞘氨醇-1-磷酸鞘氨醇（S1P）的水平。除了这些参数之外，还旨在通过不同的参数来确定可能的脂质过氧化作用。为此，在他们的同意下，从47位患者和30位健康个体中采集了血液样本和尿液。为了评估氧固醇，通过LC-MS / MS方法测量了7-酮胆固醇和胆固醇3β，5α，6β-三醇的水平。患者的7-KC含量为0.101±0.005 µmol / l，对照血浆样品为0.050±0.003 µmol / l。患者组三醇含量为0.038±0.005 µmol / l，对照组为0.033±0.004 µmol / l（p <0.001）。此外，脂质过氧化产物是通过人-8-异前列腺素，人-4-羟基壬烯（4-HNE）和人丙二醛（MDA）ELISA试剂盒测量的。患者和对照组的血浆HNE水平分别为血浆735.14±288.80 pg / ml和尿液595.72±108.62 pg / ml，尿液为606.02 + 118.23 pg / ml和531.84 + 107.18 pg / ml（p <0.05） 。患者和对照组的F2-iP结果分别为尿液450.0 + 101.40 pg / dl和386.9 + 112.7 pg / ml，血浆432.7±188,8 pg / dl和321.9±69.4 pg / dl（p <0.05）。患者的血浆MDA水平为44.1±14.6 nmol / ml，对照组为31.9±10.5 nmol / ml（p <0.05）。患者和对照组的尿液样品中MDA水平分别为30.15 + 5.06 nmol / ml和25.15 + 6.07 nmol / ml（p <0.05）。与对照组相比，患者的S1P水平降低（49.05±10.87和67.57±16.25，p <0.001）。结果不仅表明胆固醇氧化，脂质过氧化和矽肺病之间的相关性，而且还提供了对脂质在这种炎性疾病机理中的作用的更好理解。