INTRODUCTION Fine lavender and lavandin are perfume and medicinal plants originate from the South of France and are widely cultivated for their essential oils. Recently, cultivated plants suffered from a severe decline in France, due to the propagation of the yellow decline disease. This disease is caused by the stolbur phytoplasma, a bacterium transmitted by a sap-sucking insect, the planthopper. OBJECTIVES In order to understand the complex relationships between host plant, pest, pathogen and environment responsible for the yellow decline of lavender, we use a metabolomic approach to highlight changes in chemical emissions from asymptomatic ("healthy") and symptomatic ("infected") plants. METHODS Volatile compounds produced by fine lavender and lavandin were collected in the field using a dynamic headspace extraction approach. Afterwards, compounds trapped on Tenax adsorbent were thermodesorbed and analysed using an automated thermal desorption-gas chromatography-mass spectrometry (ATD-GC-MS). Multivariate statistical analyses was performed using principal component analysis and partial least square discriminant analyses. RESULTS The untargeted screening of volatiles allowed the separation of asymptomatic and symptomatic plants according to their emissions. The approach was sufficiently accurate so as to separate the emissions according to the different stages of infection. Twelve compounds were found to be deregulated metabolites of yellow disease infection, common to fine lavender (variety 7713) and lavandin (variety abrial). CONCLUSION The metabolomic approach allowed for the effective identification of chemical variations between infected and healthy plants in a complex field environment.

中文翻译：

---

在露天条件下生长的薰衣草排放的挥发性化合物的代谢组学研究：研究黄变病的潜在方法。

简介薰衣草和薰衣草精是香水，药用植物起源于法国南部，因其精油而被广泛种植。最近，由于黄色衰退病的传播，法国的栽培植物遭受了严重的衰退。这种疾病是由茎吸茎线虫引起的，该茎杆吸食性昆虫是飞虱。目的为了了解造成薰衣草黄变的寄主植物，病虫，病原体和环境之间的复杂关系，我们使用代谢组学方法来强调无症状（“健康”）和有症状（“感染”）化学物质排放的变化。植物。方法采用动态顶空萃取方法在田间收集由精细薰衣草和薰衣草素产生的挥发性化合物。然后，将捕获在Tenax吸附剂上的化合物进行热脱附，并使用自动热脱附-气相色谱-质谱（ATD-GC-MS）进行分析。使用主成分分析和偏最小二乘判别分析进行多元统计分析。结果通过对挥发物进行无针对性的筛选，可以根据无烟植物和有症状植物的排放量进行分离。该方法足够准确，可以根据感染的不同阶段来分离排放物。发现十二种化合物是黄色疾病感染的代谢失调产物，常见于精细的薰衣草（品种7713）和熏衣草素（品种abrial）。