Background

In this study, the biodegradation of N-acetyl-para-aminophenol also known as acetaminophen (APAP, paracetamol) was studied by bacterial strain Bacillus drentensis strain S1 (accession no. KY623719) isolated from sewage sample.

Results

The Bacillus drentensis strain S1 was isolated from the sewage sample using the enrichment culture method. As per our knowledge this is the first Bacillus drentensis strain reported for the degradation of APAP. In this study a 20-L batch reactor was employed for degradation of APAP. The maximum specific growth rate (μ_max) was observed at 400 mg/L concentration of APAP. The pilot-scale anaerobic batch reactor of was stable and self-buffered. The degradation in pilot-scale reactor was slow as compared to batch experiments due to fluctuation in pH and exhaustion of nutrients. Design-Expert^® software was used for optimization of conditions for APAP degradation; such as temperature (40 °C), pH (7.0), concentration of APAP (300 g/L) and agitation speed (165 rpm). The FTIR and GC–MS were used to identify the degradation metabolites. The intermediates of degradation like 2-isopropyl-5-methylcyclohexanone and phenothiazine were observed, based on these results the metabolic pathway has been predicted.

Conclusions

The optimization, kinetic, batch study and pilot study indicates the potential of Bacillus drentensis strain S1 for degradation of acetaminophen. The experimental design, optimization and statistical analysis were performed by Design Expert^® software. The optimal growth condition for Bacillus drentensis strain S1 was found to be at temperature 40 °C, pH 7, acetaminophen at concentration of 300 (mg/L) and agitation speed 165 rpm. The GC–MS and FTIR was used for identification of metabolites produced during acetaminophen degradation and the partial metabolic pathway for degradation of acetaminophen was also proposed

.

中文翻译：

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枯草芽孢杆菌S1对乙酰氨基酚降解的表征，优化和动力学研究及废水降解分析

背景

在这项研究中，N。乙酰基-对氨基苯酚（也称为对乙酰氨基酚）（APAP，对乙酰氨基酚）的生物降解是通过从污水样品中分离出的芽孢杆菌芽孢杆菌菌株S1（保藏号为KY623719）进行的。

结果

所述芽孢杆菌属drentensis菌株S1从使用富集培养方法的污水样品中分离。据我们所知，这是第一个报告的可降解APAP的芽孢杆菌。在这项研究中，采用20升间歇式反应器降解APAP。最大比生长速率（μ_最大在APAP的400 mg / L的浓度下观察到）。的中试规模厌氧间歇反应器稳定且具有自我缓冲能力。与中试相比，由于pH的波动和养分的消耗，中试规模反应器的降解速度较慢。设计专家^®该软件用于优化APAP降解条件；例如温度（40°C），pH（7.0），APAP浓度（300 g / L）和搅拌速度（165 rpm）。FTIR和GC-MS用于鉴定降解代谢物。观察到降解的中间体，如2-异丙基-5-甲基环己酮和吩噻嗪，基于这些结果，可以预测代谢途径。

结论

优化，动力学，分批研究和初步研究表明，德伦特芽孢杆菌菌株S1具有降解对乙酰氨基酚的潜力。实验设计，优化和统计分析，由设计专家进行^®软件。发现芽孢杆菌芽孢杆菌菌株S1的最佳生长条件是在40℃，pH 7，对乙酰氨基酚浓度为300（mg / L）和搅拌速度为165rpm的条件下。GC-MS和FTIR用于鉴定对乙酰氨基酚降解过程中产生的代谢产物，并提出了对乙酰氨基酚降解的部分代谢途径

。