Glycopolymers having three different pendant moieties, such as glucopyranoside, galactopyranoside, and mannopyranoside, were prepared and functionalized on the surfaces of nanoscale graphene oxide particles (GONPs). GONPs exhibited maximum absorbance at around 230 nm in the UV-vis spectrum with an average particle size of 200 nm as measured by dynamic light scattering measurements. The selectivity of pendant moieties of glycopolymer for binding with GONPs was assessed by fluorescence quenched in fluorescein isothiocyanate (FITC) conjugated GONPs. FITC interaction with GONPs led to a decrease in the fluorescence due to the resonance energy transfer and quantified the reappeared fluorescence of the FITC by glycopolymer co-functionalization with FITC-GONPs depending on the nature of pendant moieties and their respective concentrations. The binding of glycopolymers on GONPs was analyzed by Raman and UV-vis spectroscopic techniques. Glycopolymer chains with mannose pendant moieties exhibited the strongest binding affinity toward GONPs compared to the glycopolymer chains having galactose/glucose pendants. Graphical abstract

中文翻译：

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氧化石墨烯表面结构多样的糖聚合物的功能化及其通过荧光素异硫氰酸酯的荧光共振能量转移进行量化

在纳米级氧化石墨烯颗粒 (GONP) 的表面上制备并官能化了具有三种不同侧基的糖聚合物，例如吡喃葡萄糖苷、吡喃半乳糖苷和吡喃甘露糖苷。通过动态光散射测量，GONP 在紫外-可见光谱中的 230 nm 附近表现出最大吸光度，平均粒径为 200 nm。通过在异硫氰酸荧光素 (FITC) 共轭的 GONP 中淬灭荧光来评估糖聚合物的悬垂部分与 GONP 结合的选择性。由于共振能量转移，FITC 与 GONP 的相互作用导致荧光降低，并根据悬垂部分的性质及其各自的浓度，通过与 FITC-GONP 的糖聚合物共官能化来量化 FITC 重新出现的荧光。通过拉曼和紫外-可见光谱技术分析了糖聚合物在 GONP 上的结合。与具有半乳糖/葡萄糖侧链的糖聚合物链相比，具有甘露糖侧链部分的糖聚合物链对 GONP 表现出最强的结合亲和力。图形概要