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Functionalization of structurally diverse glycopolymers on graphene oxide surfaces and their quantification through fluorescence resonance energy transfer with fluorescein isothiocyanate

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Abstract

Glycopolymers having three different pendant moieties, such as glucopyranoside, galactopyranoside, and mannopyranoside, were prepared and functionalized on the surfaces of nanoscale graphene oxide particles (GONPs). GONPs exhibited maximum absorbance at around 230 nm in the UV-vis spectrum with an average particle size of 200 nm as measured by dynamic light scattering measurements. The selectivity of pendant moieties of glycopolymer for binding with GONPs was assessed by fluorescence quenched in fluorescein isothiocyanate (FITC) conjugated GONPs. FITC interaction with GONPs led to a decrease in the fluorescence due to the resonance energy transfer and quantified the reappeared fluorescence of the FITC by glycopolymer co-functionalization with FITC-GONPs depending on the nature of pendant moieties and their respective concentrations. The binding of glycopolymers on GONPs was analyzed by Raman and UV-vis spectroscopic techniques. Glycopolymer chains with mannose pendant moieties exhibited the strongest binding affinity toward GONPs compared to the glycopolymer chains having galactose/glucose pendants.

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Acknowledgments

NNMR thanks the UGC, India, for granting the research fellowships. DS thanks for the RA support received from CSIR-Network project. AVSS thanks the CSIR, India [DENOVA CSC-0205] (IICT/Pubs./2019/144).

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Author notes

  1. S. Arasaretnam

    Present address: Department of Chemistry, Faculty of Science, Eastern University, Chenkaladi, Sri Lanka

Authors and Affiliations

  1. Clinical Research Facility, CSIR- Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India

    Deepti Sharma & Marshal Dhayal

  2. Polymers and Functional Materials, Fluoro-Agrochemicals Department and Academy of Scientific & Innovative Research (AcSIR), CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Telangana, 500007, India

    N. Naga Malleswara Rao & Annadanam V. Sesha Sainath

  3. Nano-Cellular Medicine and Biophysics Laboratory, School of Biomedical Engineering, Indian Institute of Technology, Banaras Hindu University, Varanasi, UP, 221005, India

    Marshal Dhayal

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Sharma, D., Rao, N.N.M., Arasaretnam, S. et al. Functionalization of structurally diverse glycopolymers on graphene oxide surfaces and their quantification through fluorescence resonance energy transfer with fluorescein isothiocyanate. Colloid Polym Sci 298, 365–375 (2020). https://doi.org/10.1007/s00396-020-04611-w

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  • DOI: https://doi.org/10.1007/s00396-020-04611-w

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