Abstract

Keratin, as a promising substitute for tissue engineering due to its excellent biocompatibility and bioactivity, is used to combine one or more other polymers together. However, compound nanofibers with high keratin content (normally >90 wt.%) may result in the poor elongation of nanofiber membranes such as wound dressing. In this work, different ratios of hydroxyapatites (HA) modified by sodium hexametaphosphate were blended with keratin/polyethylene oxide (PEO) spinning solution to produce reinforced keratin blend nanofiber nonwoven membranes as a potential candidate wound dressing. The tensile strength of keratin blend nanofiber membrane with 15 % modified HA addition was two times higher than that without HA. The morphologies and chemical structure of keratin/PEO/HA nanofiber membranes were investigated using SEM, FTIR, and TG. The biocompatibility and the burn repairing performance of keratin/PEO/HA nanofiber mat were also investigated by cell culture and animal burn model. The results showed that the Keratin/PEO/HA nanofiber membrane was beneficial to enhance the proliferation of L929 cell, exhibiting an advantages in reducing inflammatory response in the infective stage and enhancing skin repairing process in the following recover stages. Our data suggested that keratin/PEO/HA nanofiber membrane could serve as a promising burn dressing for treatment of the skin burn.

中文翻译：

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具有改善的机械性能的角蛋白/ PEO /羟基磷灰石纳米纤维膜，可用于潜在的烧伤敷料应用

摘要

角蛋白由于其优异的生物相容性和生物活性而成为有希望的组织工程替代品，可用于将一种或多种其他聚合物结合在一起。但是，具有高角蛋白含量（通常> 90 wt。％）的复合纳米纤维可能导致纳米纤维膜的伸长率较差，例如伤口敷料。在这项工作中，将不同比例的六偏磷酸钠改性的羟基磷灰石（HA）与角蛋白/聚环氧乙烷（PEO）纺丝溶液共混，以生产增强的角蛋白共混纳米纤维非织造膜，作为潜在的候选伤口敷料。加入15％改性HA的角蛋白共混纳米纤维膜的抗张强度是没有HA的抗张强度的两倍。使用SEM，FTIR和TG研究了角蛋白/ PEO / HA纳米纤维膜的形貌和化学结构。还通过细胞培养和动物烧伤模型研究了角蛋白/ PEO / HA纳米纤维垫的生物相容性和烧伤修复性能。结果表明，角蛋白/ PEO / HA纳米纤维膜有利于增强L929细胞的增殖，在减少感染阶段的炎症反应和在后续恢复阶段增强皮肤修复过程方面具有优势。我们的数据表明，角蛋白/ PEO / HA纳米纤维膜可以用作治疗皮肤烧伤的有前途的烧伤敷料。在减少感染阶段的炎症反应以及在随后的恢复阶段增强皮肤修复过程方面显示出优势。我们的数据表明，角蛋白/ PEO / HA纳米纤维膜可以用作治疗皮肤烧伤的有前途的烧伤敷料。在减少感染阶段的炎症反应以及在随后的恢复阶段增强皮肤修复过程方面显示出优势。我们的数据表明，角蛋白/ PEO / HA纳米纤维膜可以用作治疗皮肤烧伤的有前途的烧伤敷料。