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Keratin/PEO/hydroxyapatite Nanofiber Membrane with Improved Mechanical Property for Potential Burn Dressing Application

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Abstract

Keratin, as a promising substitute for tissue engineering due to its excellent biocompatibility and bioactivity, is used to combine one or more other polymers together. However, compound nanofibers with high keratin content (normally >90 wt.%) may result in the poor elongation of nanofiber membranes such as wound dressing. In this work, different ratios of hydroxyapatites (HA) modified by sodium hexametaphosphate were blended with keratin/polyethylene oxide (PEO) spinning solution to produce reinforced keratin blend nanofiber nonwoven membranes as a potential candidate wound dressing. The tensile strength of keratin blend nanofiber membrane with 15 % modified HA addition was two times higher than that without HA. The morphologies and chemical structure of keratin/PEO/HA nanofiber membranes were investigated using SEM, FTIR, and TG. The biocompatibility and the burn repairing performance of keratin/PEO/HA nanofiber mat were also investigated by cell culture and animal burn model. The results showed that the Keratin/PEO/HA nanofiber membrane was beneficial to enhance the proliferation of L929 cell, exhibiting an advantages in reducing inflammatory response in the infective stage and enhancing skin repairing process in the following recover stages. Our data suggested that keratin/PEO/HA nanofiber membrane could serve as a promising burn dressing for treatment of the skin burn.

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Acknowledgements

The authors gratefully acknowledge financial support by the National Natural Science Foundation of China (Grant No. 51573133) and Natural Science Foundation of Ningbo (No. 2018A610104).

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Authors and Affiliations

  1. State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin, 300387, China

    Jie Fan & Yong Liu

  2. School of Textile Science and Engineering, Tianjin Polytechnic University, Tianjin, 300387, China

    Jie Fan, Tongda Lei, Mengyan Yu & Yong Liu

  3. Medical Experimental Center, North China University of Science and Technology, Tangshan, 063210, China

    Yongheng Wang

  4. Laboratory Animal Center, North China University of Science and Technology, Tangshan, 063210, China

    Fuyuan Cao

  5. Dermatological Department, Air Force General Hospital, Beijing, 100142, China

    Qingqi Yang

  6. Medical Research Center, North China University of Science and Technology, Tangshan, 063210, China

    Faming Tian

Authors
  1. Jie Fan
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  2. Tongda Lei
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  3. Mengyan Yu
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  4. Yongheng Wang
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  5. Fuyuan Cao
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  6. Qingqi Yang
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  7. Faming Tian
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  8. Yong Liu
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Correspondence to Jie Fan or Yong Liu.

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Fan, J., Lei, T., Yu, M. et al. Keratin/PEO/hydroxyapatite Nanofiber Membrane with Improved Mechanical Property for Potential Burn Dressing Application. Fibers Polym 21, 366–375 (2020). https://doi.org/10.1007/s12221-020-9406-x

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  • DOI: https://doi.org/10.1007/s12221-020-9406-x

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